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FlowPublic

FlowPublicTM is a free repository of validated technical cytometry and computer resources.

Table Of Contents

Vendor-Specific

BD Biosciences

FACSDiva Software

WORK-FLOW Diva Manager
A lightweight app to keep your installation healthy by managing CS&T results, performing database replacements, and more.

Java Runtime Environment 6u29
FACSDiva 8 JRE version.

FACSDiva LJ Importer
Microsoft Excel Macro to import, parse, and graph FACSDiva Levey-Jennings data.

FACSDiva 8.0.3 Standalone (analysis) Installation
How to install FACSDiva 8.0.3 on a Windows 10 64-bit PC not connected to an instrument.

FACSDiva 9.0 Standalone (analysis) Installation
How to install FACSDiva 9.0 on a Windows 10 64-bit PC not connected to an instrument.

FACSDiva Errors & Quirks
How to fix several FACSDiva errors and unexpected behaviors.

FACSDiva Database Relocation
How to relocate the FACSDiva database from the D drive to the C drive.

FACSDiva Configuration Modifications
How to add parameters to a configuration with CS&T results.

FACSDiva User Preferences Modifications
How to modify the CS&T user preferences.

FACSDiva Levey-Jennings Mismatch
How to fix a Levey-Jennings mismatch error.

FACSDiva Missing Compensation Control Plots
How to address missing compensation control plots in FACSDiva.

FACSDiva CS&T Results File Corruption
How to fix a corrupted CS&T results file.

FACSDiva CS&T User Preferences File Corruption
How to fix a corrupted CS&T user preferences file.

FACSDiva Cell Sorters – Camera Settings Adjustment
A guide to adjust camera settings on FACSDiva-based cell sorters.

Unlocking FACSDiva Spectral Plots
A guide to activate spectral functions on non-spectrally enhanced instruments.

FACSDiva SE Voltage Adjustment
A script to adjust voltage values of an entire detector array by a specific percentage to maintain spectral signatures on SE instruments.

FACSChorus Software

FACSChorus Instrument Service Restart
Script to restart the BD FACSChorus Instrument Service and establish an instrument connection (FACSDiscover S8).

FACSAria

FACSAria FSC PMT Option
How to use the FSC PMT option on a FACSAria.

FACSAria II Static Plumbing
Plumbing schematics of the FACSAria II cell sorter.

FACSAria Nozzle Repair Tutorial
How to repair a FACSAria nozzle tip (Protocol w/ video link).

FACSCalibur

FACSCalibur Pressure Gauge Installation
How to add a pressure gauge to a FACSCalibur.

FACSymphony

FACSymphony Connectivity Issues
How to visualize the internal computer’s output, adjust the system date and time, replace the CMOS battery, and assess communication through network activity.

FACSVantage

FACSVantage LASER Alignment Procedures
Old school BD video.

LSRFortessa (X-20)

LSRFortessa (X-20) Connectivity Issues
How to replace the CMOS battery and assess communication through network activity.

FlowJo Software

FlowJo Script Editor
How to use the FlowJo Script Editor to perform calculations, retrieve, and generate keywords.

FlowJo Plate Editor Bridge
A macro to generate a FlowJo Plate Editor-ready csv file from an Excel document.

General

LASER Communication Port Settings & Tips
Instructions to adjust COM port settings and tips to fix Coherent Connection issues.

Fluidics Cart Level Sensor Bypass
Instructions to bypass a fluidics cart defective sensing probe.

Beckman Coulter

CyAn ADP

CyAn ADP Static Plumbing
Plumbing schematics of the CyAn ADP analyzer.

CyAn ADP SMS Technical Information
SMS control board layout and SMS fluidic state chart.

CyAn ADP Flow Cell Flush
Procedure to flush the flow cell of a CyAn ADP analyzer.

Summit Settings Corruption
Guide to fix an “unknown” Summit error (Registry settings corruption).

MoFlo

MoFlo LASER Upgrade
Expanding the MoFlo Legacy/XDP capabilities with easy LASER swapping (Poster 241 – CYTO 2016).

Cytek Biosciences

Amnis ImageStreamX

ISX Spatial Offset Procedure
What to do in case of a failing Spatial Offsets Calibration in ASSIST.

IDEAS Software

Creating Histogram Overlays
How-to guide from Amnis on how to create histogram overlays from a single file or from multiple files.

SpectroFlo

SpectroFlo Warmup Bypass
A script to bypass SpectroFlo’s 30-minute warmup cycle.

Sartorius

ForeCyt Software

ForeCyt Data Store Fix
Script to start the IntelliCyt Service and fix the “ForeCyt data store” error.

HyperCyt

HyperCyt GSIOC Server Error
How to fix a GSIOC Server error on the HyperCyt system.

iQue

iQue Data Management
A guide to manage data generated by the Sartorius IntelliCyt iQue analyzers.

Sony Biotechnology

SH800/MA900 Cell Sorter Software

SH800-MA900 Cell Sorter Software – Failure to Launch
A guide to resolving Microsoft SQL Server startup issues to fix a non-starting Cell Sorter Software.

Thermo Fisher Scientific

Attune NxT

Attune NxT Service Logs & File Paths
Paths to the Attune NxT logs (System and USB), PT results, and user data.

General

Droplet Volume Estimations
A guide to estimate the volume of a sorted droplet using several methods.

Sort Calculator v1.0
Excel-based sort calculator to approximate sort timing and collection vessel requirements.

Spectral nOverlay
Excel-based normalization tool to display and compare spectral signatures.

FCS Multi-File Version Modifier
A script to modify the FCS version of files in bulk.

Computer

Operating System – Microsoft Windows

Data Management

Data Management
How to manage data when dealing with multiple windows accounts and when working with Microsoft OneDrive storage solution.

Live Drive Partition Resizing
A guide to reallocate space between 2 partitions (C/D) without the need to reformat the drive.

Robocopy Incremental Data Backup & Cleaning
How to perform automatic incremental data backups and cleaning using Microsoft Robocopy.

Optimization

Windows Optimization
How to modify various Windows 7 and Windows 10 settings; useful when dealing with multiple user accounts.

Miscellaneous

Locating a Computer’s Hardware Address
A quick guide to find your hardware address on a PC or a Mac.

Hardware

Storage

Solid-State Drive Installation (SATA)
How to upgrade from a hard disk drive to a SATA solid-state drive.

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Be sure to read the instructions provided with apps & scripts before use and visit our FAQ if you would like to contribute.