Mitch Guttman (@mitchguttman) / X

Mitch Guttman

325 posts

Mitch Guttman

@mitchguttman

Molecular biologist interested in non-coding RNAs, nuclear organization, and gene regulation. Professor at Caltech

Los Angeles, CA

guttmanlab.caltech.edu

Joined May 2015

Pinned
Mitch Guttman
@mitchguttman
Nov 27, 2024
Gene regulation involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. Our paper in @NatureGenet describes ChIP-DIP, a method for genome-wide mapping of hundreds of DNA-protein interactions in a single experiment.
ChIP-DIP maps binding of hundreds of proteins to DNA simultaneously and identifies diverse gene...
From nature.com
58K
Mitch Guttman
@mitchguttman
Nov 4, 2021
What drives organization of RNA, DNA, and proteins in the nucleus? In our new paper out @CellCellPress, we show that non-coding RNAs play a central role through a unique RNA-based mechanism. authors.elsevier.com/c/1e132L7PXf05J
Mitch Guttman
@mitchguttman
Dec 19, 2023
Gene expression involves thousands of proteins that bind DNA, yet comprehensively mapping these is challenging. We developed ChIP-DIP – a method for simultaneous, genome-wide mapping of hundreds of DNA-protein interactions in a single experiment. biorxiv.org/content/10.110…
107K
Mitch Guttman
@mitchguttman
Jun 9, 2023
Many proteins bind RNA, yet we still don’t know what RNAs most RBPs bind because current methods map one protein at a time. With the Jovanovic lab, we describe SPIDR – a method for mapping the RNA binding sites of dozens of RBPs in a single experiment. biorxiv.org/content/10.110…
82K
Mitch Guttman
@mitchguttman
Feb 22, 2024
PRC2 is reported to bind RNA. Yet, genetic experiments have raised serious questions about their functional relevance. Our paper revisits the biochemical evidence supporting widespread PRC2-RNA interactions and finds that many of these do not occur in vivo.
107K
Mitch Guttman
@mitchguttman
Jan 6, 2023
What are the functional roles of 3D genome organization? In a new preprint led by @_prashantbhat, we demonstrate a critical role for dynamic 3D organization of genomic DNA around nuclear speckles in controlling the efficiency of mRNA splicing. biorxiv.org/content/10.110…
100K
Mitch Guttman
@mitchguttman
Nov 11, 2021
Excited to see our paper featured on the cover of the current issue of Cell (@CellCellPress)! On the cover, RNAs scaffold, recruit, and place different building blocks of the nucleus. Artwork by Inna-Marie Strazhnik (@Wonderstrucksci)
Mitch Guttman
@mitchguttman
Nov 4, 2021
What drives organization of RNA, DNA, and proteins in the nucleus? In our new paper out @CellCellPress, we show that non-coding RNAs play a central role through a unique RNA-based mechanism. authors.elsevier.com/c/1e132L7PXf05J
Mitch Guttman
@mitchguttman
Mar 26, 2022
Why are lncRNAs lowly expressed? How can they regulate their more abundant targets? Our paper published in @NatureSMB we explore how these features are balanced to ensure target specificity and robust gene regulation via a spatial amplification mechanism.
Xist spatially amplifies SHARP/SPEN recruitment to balance chromosome-wide silencing and specific...
From nature.com
Mitch Guttman
@mitchguttman
May 8, 2024
What are the functional roles of 3D genome organization? Our paper in @Nature led by @_prashantbhat demonstrates how dynamic 3D genome organization around nuclear speckles plays a crucial role in regulating mRNA splicing efficiency. rdcu.be/dHkez
80K
Mitch Guttman
@mitchguttman
Nov 30, 2022
Do specific locations in the nucleus preclude transcription? In a new paper led by @sofiquinodoz & Isabel Goronzy, we show that Pol II txn occurs throughout the nucleus, including locations thought to be inactive (B compartments & nucleoli). tinyurl.com/25ueavsp
Mitch Guttman
@mitchguttman
Oct 30, 2021
Why are lncRNAs lowly expressed? How can they regulate their more abundant targets? In our new preprint we explore how these features are balanced to ensure target specificity and robust gene regulation via a spatial amplification mechanism (biorxiv.org/content/10.110…)
biorxiv.org
Xist spatially amplifies SHARP recruitment to balance chromosome-wide silencing and specificity to...
Although thousands of lncRNAs are encoded in mammalian genomes, their mechanisms of action are largely uncharacterized because they are often expressed at significantly lower levels than their...
Mitch Guttman
@mitchguttman
May 20, 2022
Really proud of @jimmykguo for giving an awesome talk on his graduate work at CSHL Regulatory RNA meeting - his first at a major meeting! #cshlregRNA
Mitch Guttman
@mitchguttman
Sep 13, 2023
Excited to be co-organizing the 2023/2024 Keystone Regulatory RNA meeting in Banff. We have an amazing lineup of speakers #KSRegRNA24. Join them by submitting an abstract for consideration by 9/25/2023. keysym.us/KSRegRNA24
17K
Mitch Guttman
@mitchguttman
Jan 11, 2022
Interested in doing SPRITE? Checkout our detailed protocol just published @NatureProtocols (nature.com/articles/s4159…) along with a video protocol (youtu.be/6SdWkBxQGlg)
Sofi Quinodoz
@sofiquinodoz
Jan 10, 2022
Want to generate higher-order 3D maps of DNA organization? Our protocol for DNA-SPRITE is now live on @NatureProtocols! Good for genomics pros and newbies alike, this walks you through SPRITE step-by-step. nature.com/articles/s4159…
SPRITE: a genome-wide method for mapping higher-order 3D interactions in the nucleus using combin...
From nature.com