Education
FlowEssentialsTM is an on-demand, streamlined, brand-neutral training program, delivered as subtitled video-based micro-content and simulation.
Course Details
Modern and cost-effective, FlowEssentialsTM eliminates the tedious and time-consuming theoretical classes usually employed by core facilities and guarantees that all users receive the same quality information on common concepts and practical considerations before attending an instrument-specific session.
Approved users can create their own account and immediately start taking the course anywhere and at their own pace.
Beginners can stop and rewind the videos to fully understand the concepts or revisit the illustrations. Advanced users needing a refresher or required to take this course will make efficient use of their time thanks to the optimized delivery.
A total of 75 quiz questions, evenly allotted at the end of each section, help ascertain knowledge validation prior to the obtention of a certificate of completion.
An extensive glossary and access to the FlowHub, our resources platform, are available to complement the learning process.
1 – Principles & Instrumentation
What is Flow Cytometry?
Common Applications
Fluorescence – Light Spectrum
Fluorescence – Absorption & Emission
Fluorescence – Fluorochrome Classes
Fluorescence – Fluorochrome Properties
Fluidics – Flow
Fluidics – Pressure & Flow Rate
Fluidics – Acoustic-Assisted Focusing
Optics – Excitation
Optics – Light Collection
Optics – Photodetection
Electronics – Amplification & Conversion
Electronics – Voltage Pulse
Electronics – Time Delay
Electronics – Digitization & Binning
Electronics – FCS File
Section Summary
2 – Reagents & Panel Design
Sample Requirements
Cellular Markers and Dyes
Fluorescence Detection
Fluorochrome Selection
Antigen Classification
Antibody Titration
Dead Cell Staining
Exclusion / Dump Channel
Spectral Overlap
Compensation Controls
Compensation
Spectral Overlap Reduction
Spillover Spreading
Spillover Spreading Matrix
Autofluorescence
Fc-Mediated Binding
Fluorescence Minus One Controls
Biological Controls
Replicates
Section Summary
3 – Acquisition & Measurements
Plots & Gates
Scaling
Acquisition Speed
Event Cycling
Scatter Setup
Threshold & Trigger
Aggregate Discrimination
Dead Cell Exclusion
Fluorescent Parameter Setup
Compensation
Data Recording
Data Exporting
Assay Setup Validation
Instrument Cleaning
Section Summary
4 – Analysis (with Interactive Tools)
Matrix Inspection
Data Display – Histograms & Plots
Data Display – Gates, Regions, Quadrants
Data Display – Gating
Biexponential Scaling
Data Cleaning – Time Gating
Data Cleaning – Debris Exclusion
Data Cleaning – Aggregates & Dead Cells
Data Cleaning – Autofluorescence
Data Cleaning – Antibody Aggregates
Data Cleaning – Impact
Gate Validation – FMO Controls
Gate Validation – Backgating/Color Gating
Cell Cycle – One-color DNA Analysis
Cell Cycle – Multi-color DNA Analysis
Section Summary
Interactive Tools
Matrices
Plotting
Gating
Scaling
Cleaning Impact
Controls
DNA Analysis
5 – Statistics
Number of Events to Record
Descriptive Statistics – Distribution
Descriptive Statistics – Central Tendency
Descriptive Statistics – Dispersion
Descriptive Statistics – Data Normalization
Descriptive Statistics – Fold Change
Inferential Statistics – Statistical Test
Inferential Statistics – Statistical Power
Inferential Statistics – Hypotheses
Inferential Statistics – Significance Level
Inferential Statistics – Statistical Errors
Inferential Statistics – Probability Value
Student’s t-test – T-distribution & Freedom
Student’s t-test – Formula
Student’s t-test – Real-Life Example pt1
Student’s t-test – Real-Life Example pt2
Section Summary
Access Instructions
- Already registered? Click LOGIN then the Access top menu | Not yet registered? Click REGISTER on the top menu (instructions)
- Group leader? Access the User Management Dashboard (instructions)
FlowAssaysTM is a course designed to guide users through the diverse capabilities of flow cytometry.
Course Outline
Introduction
Surface Antigen Staining
Intracellular Staining
Reporter Proteins
Cell Cycle
Cell Proliferation
Cell Viability
Cellular Senescence
Cell Death
Reactive Oxygen Species
Intracellular Ion Measurements
Intracellular pH
Internalization
Stem Cell Detection
Chromosome Characterization
Multiplex Immunoassays
RNA Detection
FRET
Signal Amplification
Receptor Occupancy Assays
Small Particles
Clinical Applications
Final Considerations
Access Instructions
- Already registered? Click LOGIN then the Access top menu | Not yet registered? Click REGISTER on the top menu (instructions)
- Group leader? Access the User Management Dashboard (instructions)
FlowSelectTM is a series of cytometry courses on advanced, specialty topics.
Cell Sorting
Course Outline
What is Cell Sorting? | Process Overview
Nozzle Size & Sheath Pressure | Droplet Generation
Droplet Breakoff
Stream Charging & Deflection
Collection
Instrument Optimization | Drop Delay
Acquisition & Gating
Conflicts
Sort Decisions
Sorting
Post-Sort – Purity, Recovery & Yield
Process Stressors | Sample Quality
Temperature & Pressure | Sample Media & Sheath
Collection Media | Instrument Variables
Sort Performance | Sort Quality
Collection Issues | Contamination
Single-Cell Deposition
Biosafety | Summary
Quiz (20 questions)
Full-Spectrum Flow Cytometry + Panel Optimization
Course Outline
What is Full-Spectrum Flow Cytometry?
Full-Spectrum vs. Conventional
Spectral Emission Signature
Signature Comparison
Signature Alterations
Reference Controls
Spectral Reference Library
Instrument Settings
Acquisition
Spectral Profile & Unmixing
Autofluorescence Management
Unmixing Errors
Analysis
Panel Optimization – Introduction
Panel Optimization – Design
Panel Optimization – Testing
Panel Optimization – Evaluation & Remediation
Summary
Quiz (20 questions)
Access Instructions
- Already registered? Click LOGIN then the Access top menu | Not yet registered? Click REGISTER on the top menu (instructions)
- Group leader? Access the User Management Dashboard (instructions)
FlowEvalTM is a proprietary flow cytometry knowledge assessment system.
Contents
FlowEval is a comprehensive evaluation and preparation resource designed to test and expand your knowledge of flow cytometry. Whether you are studying for an exam or refining your expertise, FlowEval offers more than 1,000 questions and answers, with explanations for 100% of the entries.
Beyond assessment, FlowEval also provides practical considerations, guidance on best troubleshooting practices, and essential operational procedures, ensuring a well-rounded understanding of both theory and hands-on techniques.
Key Features:
• Extensive Question Bank: Access a wide range of flow cytometry questions
• Detailed Explanations: Understand the “why” behind the answers, with added insights for further clarity
• Realistic Practice Exams: Simulate exam conditions with full-length practice tests
• Access Anywhere: Study on-the-go with our responsive and user-friendly platform
Who Can Benefit?
• Anyone seeking to enhance and validate their flow cytometry knowledge
• Students preparing for flow cytometry coursework and exams (i.e., ESCCA European Certificate for Cytometry Operators exam and Specialist in Cytometry, SCYM(ASCP) exam)
Question Categories:
– Instrumentation: Fluidics (53) | Optics (69) | Electronics (51) | Troubleshooting & Cleaning (56)
– Experimentation: Sample (94) | Assay Development (111) | Clinical Applications (120) | Research Applications (53)
– Data: Generation (80) | Analysis (81)
– Laboratory Operations: Quality Control (40) | Assay Validation (58) | Safety (68) | Administration (97)
Preview
Access
- Already registered? Click LOGIN then the Access top menu | Not yet registered? Click REGISTER on the top menu (instructions)