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Super excited to share our recent work now out in @Nature! We introduce trans-synaptic tracing tools from circuit neuroscience to study the whole-brain connectome of glioblastoma. nature.com/articles/s4158…
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Yusha Sun
72 posts
Yusha Sun
@sun_yusha
MD/PhD student @PennMedicine @UPenn_SongMing | @Princeton @PrincetonPhys alum | Interested in neuro-oncology, cancer neuroscience, single-cell genomics
- Thrilled to share our new preprint! We leverage GBM organoids and monosynaptic rabies tracing to reveal the brain-wide connectome of this cancer, including a cholinergic neuron-tumor synapse that may fuel GBM invasion. @UPenn_SongMing #CancerNeuroscience
- Our recent work on the use of glioblastoma organoids (GBOs) to assess CAR-T clinical trial responses in real time together with @LogunMeghan @XinW_up @StephenBagleyMD @UPenn_SongMing @DrORourke2 is now online @CellStemCell!
- Replying to @sun_yushaThis has been an incredible journey and I am so grateful for my mentors @UPenn_SongMing, an amazing team in the lab @XinW_up, @danzhang122, Zhijian, @kristenhpark and many more. Special thanks to @minghongmaUPenn, @DrORourke2, @MacNasrallah @EmilyllPai @PennMedicine
- Replying to @sun_yushaWe hope this study provides new tools for the study of #CancerNeuroscience and may eventually lead to a better understanding of this devastating disease.
- Replying to @sun_yushaMonosynaptic herpes simplex virus tracing, immuno-electron microscopy, and functional assays all confirm a cholinergic neuron-to-glioma synapse. Acetylcholine may play a role in GBM invasion via the CHRM3 receptor, which is a potential therapeutic target.
- Replying to @sun_yushaBy transplanting glioblastoma organoids and performing monosynaptic rabies virus tracing, we find rapid and extensive brain-wide circuit integration of GBM. GBM inputs are highly diverse and include, for example, long-range cholinergic neurons from the basal forebrain.
- Replying to @sun_yushaThanks a ton to our GBO team, including @XinW_up @danzhang122 @kristenhpark and our collaborators, including @minghongmaUPenn @DrORourke2 @MacNasrallah and many others at @PennMedicine and beyond!
- Replying to @sun_yushaWe generated GBOs in tandem with our phase 1 trial of bivalent CAR-T cells in recurrent GBM (doi.org/10.1038/s41591…) and treated them with patient CAR-T cells. We found that cytolysis and cytokine release mirrored patient responses, highlighting the potential of this platform.
- Check out the preprint for our new study on the temporal dynamics of CAR-T cells with GBM organoids, mapped in exquisite detail! Congrats @danzhang122!!So excited to share this study looking at the dynamic interplay between GBM and CAR T cells during treatment using organoids and single cell multi-omics! Hopefully this moves us toward a better understanding of #immunotherapy in #GBM. Huge thanks to @UPenn_SongMing @DrORourke2!
- Replying to @sun_yushaWe thank the invaluable contributions of the entire team @MacNasrallah @danzhang122 @EmilyllPai @PennMedicine and many others!
- Replying to @sun_yushaHere is a direct link: authors.elsevier.com/c/1kEhZ6tu0Cpv…
