Han Altae-Tran (@altaetran) / X

Han Altae-Tran

93 posts

Han Altae-Tran

@altaetran

Fascinated by all things science. Protein designer (aka postdoc) at UW | PhD BE, MIT | MS EE, BS Math, Physics, Stanford

University of Washington

Joined August 2021

Pinned
Han Altae-Tran
@altaetran
Nov 28, 2023
Our latest work exploring ultra rare CRISPR systems with new big data algorithms has been published by @ScienceMagazine! @zhangf @soumya_kannan12. This thread will unwrap how we found needles in a haystack of metagenomes. Let's dive in! (1/8) doi.org/10.1126/scienc…
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Han Altae-Tran
@altaetran
Dec 3, 2023
Our latest research, published in @PNASNews, identifies nearly 1 million OMEGA-TnpB & CRISPR-Cas12 genome editors. Key insights? RNA-guided activity is linked to more processes than we thought: transcription, transposition, motility and more! doi.org/10.1073/pnas.2… #CRISPR (1/3)
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Han Altae-Tran
@altaetran
Nov 25, 2023
Check out our latest work on a new algorithm for protein clustering and the discovery of new CRISPR systems! More on this soon. So grateful to have this work published by @ScienceMagazine !
Science Magazine
@ScienceMagazine
Nov 23, 2023
Using a new algorithm called FLSHclust, researchers have revealed rare and previously unknown #CRISPR-Cas systems. The approach and results provide novel opportunities for understanding the vast functional diversity of microbial proteins. scim.ag/4W6
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Han Altae-Tran
@altaetran
Nov 30, 2023
CRISPR defense now known to target mRNAs in ribosomes - the source of all proteins in the cell - in order to prevent viruses from making proteins. Truly amazing!
science.org
Ribosomal stalk-captured CARF-RelE ribonuclease inhibits translation following CRISPR signaling
A prokaryotic antiviral CRISPR-Cas auxiliary effector cleaves ribosome-bound messenger RNAs to inhibit translation
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Han Altae-Tran
@altaetran
Sep 9, 2021
Excited to share our latest work uncovering new classes of RNA-guided systems! @soumya_kannan12 @zhangf
Feng Zhang
@zhangf
Sep 9, 2021
CRISPR technologies are powerful gene editing tools because they are programmable. We are excited to describe 3 new programmable systems, IscB, IsrB, and TnpB, some of the most abundant genes on the planet, with potential for gene editing. @ScienceMagazine science.org/doi/10.1126/sc…
Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
🌐 With now over a billion sequenced known proteins, it has become challenging to analyze them all. So we developed FLSHclust, an algorithm that can deep cluster billions of proteins in nearly linear time. #BigData #Bioinformatics (2/8)
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Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
🔍 Strikingly, a candidate Type VII CRISPR system emerged. We show that these are a new class of RNA editors that rely on a new effector, Cas14, which contains a β-CASP RNase domain and a domain distantly related to part of Cas10. (4/8)
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Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
We're excited about the potential impact of our work and look forward to collaborating with the scientific community to explore these systems further. A huge thank you to our team and collaborators @rachel_oshiro @lkmoeller. Follow us for more updates #TeamScience (8/8)
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Han Altae-Tran
@altaetran
Dec 5, 2023
Navigating grad school apps? I've been there! Offering help for anyone diving into Bio, Bioengineering, and Bioinformatics PhD applications. Happy to share feedback & some much-needed moral support. Let's make this journey easier! #PhD #GradSchoolApps
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Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
🧬 The remaining systems we identified include new CRISPR-linked transposon systems, CARF effectors, reverse-transcriptase associated acquisition modules, large ATPase systems, and more, highlighting the vast untapped natural diversity of CRISPR functions #CRISPR (7/8)
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Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
🔍 The functions of type IV CRISPR systems have remained enigmatic for years. Our search unveiled a new subtype that uses an HNH nuclease domain, resulting in targeted processive degradation of DNA (5/8)
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Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
🌐 We used FLSHclust to develop an ultra-sensitive CRISPR discovery pipeline, discovering 188 rare CRISPR-linked systems from ~8TB of genomic data. These systems we found have diverse biochemical functions. #GenomeMining #CRISPR (3/8)
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Han Altae-Tran
@altaetran
Nov 28, 2023
Replying to @altaetran
🔍We also uncovered new types of precise genome editors in which multi-component type I CRISPR systems naturally lost Cas3 and gained an HNH nuclease, resulting in newly evolved precise genome editors. #GenomeEditing (6/8)
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Han Altae-Tran
@altaetran
Dec 1, 2023
A new preprint from @shsternberg @SternbergLab shows that TnpB, the ancestor of CRISPR-Cas12, has been naturally co-opted for RNA-guided transcriptional regulation of bacterial flagellin systems! doi.org/10.1101/2023.1…
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