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Alice Ting
@aliceyting
Molecular designer
Stanford, CA
tinglab.org
Joined June 2017
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  • user avatar
    Alice Ting
    @aliceyting
    Jun 28, 2023
    TransitID is out today in Cell! This is a proximity labeling method for unbiased discovery of endogenous proteins that traffick from a defined “source” location to a defined “destination” location. TransitID uses sequential labeling by TurboID in the source, followed by a chase
    284K
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    Alice Ting
    @aliceyting
    May 3, 2023
    I was taken by surprise this morning when congratulatory emails started arriving in my inbox. I'm incredibly honored, grateful, and humbled to be included among such an esteemed group of scientists, many of whom I’ve admired for decades. While this recognition is individual,
    222K
  • user avatar
    Alice Ting
    @aliceyting
    Jan 11, 2021
    We wrote a proximity labeling (PL) review to highlight all the exciting applications of PL to protein-protein & protein-RNA/DNA interaction mapping. See examples of PL applied to enzyme-substrate interactions, insoluble baits, dynamic PPI networks & multifunctional proteins.
  • user avatar
    Alice Ting
    @aliceyting
    Dec 4, 2024
    Could one envision a synthetic receptor technology that is fully programmable, able to detect diverse extracellular antigens – both soluble and cell-attached – and convert that recognition into a wide range of intracellular responses, from transgene expression and real-time
    125K
  • user avatar
    Alice Ting
    @aliceyting
    Dec 16, 2022
    Can we perform unbiased discovery of endogenous proteins that traffick from one organelle to another in living cells? Learn about our new proximity labeling method, TransitID, here: Full video of ASCB lecture at tinyurl.com/3dste3am
    00:00
    108K
  • user avatar
    Alice Ting
    @aliceyting
    Sep 26, 2019
    Huimin Zhao shared this hilarious 1998 abstract on the “Directed Evolution of a Full Professor” at the #LSIsymposium2019. “A pool of grad student clones was [selected] on minimal money media & the ability to work long hours w/vending machine snacks as a sole carbon source.”
  • user avatar
    Alice Ting
    @aliceyting
    Apr 15, 2024
    Deeply saddened by the passing of Lubert Stryer, father of FRET, pioneer of DNA and peptide microarrays, author of the famous Biochemistry textbook used across the world. Many may not know that he was also an extremely generous supporter of the arts and deeply passionate about
    95K
  • user avatar
    Alice Ting
    @aliceyting
    Mar 10, 2023
    Light-regulated TurboID, or LOV-Turbo, is a feat of engineered allostery, created by @SongyiLee10 and @CheahJoleen via structure-guided design, then optimized by yeast display directed evolution. Activity is nearly undetectable in the dark state, but biorxiv.org/cgi/content/sh…
    220K
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    Alice Ting
    @aliceyting
    Oct 31, 2024
    APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2 is a liability. We now report LaccID, a multicopper oxidase evolved from an ancestral fungal laccase that oxidizes aromatic substrates using non-toxic O2
    65K
  • user avatar
    Alice Ting
    @aliceyting
    Sep 13, 2021
    First time giving a talk in a baseball stadium, with slides displayed on a jumbotron! At the Stanford Genetics retreat, where organizers have been creative & working hard to put on a safe in-person event *Retreat swag included baseball Tshirts and double helix baseball caps!
  • user avatar
    Alice Ting
    @aliceyting
    May 15, 2023
    LOV-Turbo is published today in @naturemethods and plasmids for LOV-Turbo are available on Addgene, here: addgene.org/browse/article… We hope many people will use LOV-Turbo in exciting new ways! nature.com/articles/s4159… If you plan to use NanoLuc BRET to activate LOV-Turbo, please
    user avatar
    Alice Ting
    @aliceyting
    Mar 10, 2023
    Light-regulated TurboID, or LOV-Turbo, is a feat of engineered allostery, created by @SongyiLee10 and @CheahJoleen via structure-guided design, then optimized by yeast display directed evolution. Activity is nearly undetectable in the dark state, but biorxiv.org/cgi/content/sh…
    148K
  • user avatar
    Alice Ting
    @aliceyting
    Nov 2, 2020
    We’ve written a detailed step-by-step protocol for how to use TurboID (& split-TurboID) in cultured mammalian cells! How to select your proximity labeling (PL) enzyme, design & execute PL experiments, & analyze the resulting mass spec data. tinyurl.com/y2rrftu6
  • user avatar
    Alice Ting
    @aliceyting
    Jun 22, 2021
    To improve spatial and temporal control over proximity labeling, we’ve developed LOV-Turbo, a reversible & blue-light activated variant of TurboID. Almost no dark state leak & +light activity comparable to miniTurbo. We are doing pulse-chase labeling with LOV-Turbo to map..
  • user avatar
    Alice Ting
    @aliceyting
    Oct 9, 2024
    In my several decades in science, few moments have been as seismic as when AlphaFold burst onto the scene (seeing the first structure of a K+ ion channel, from Rod MacKinnon, was up there too). We rushed to fold every protein we had been working on, that had no structure, and
    33K