Advanced protein stability, made simple
Plate-based intrinsic DSF (differential scanning fluorimetry) delivers clearer stability insights faster, more affordably, and directly in your standard microplate workflow.
SUPR-DSF is a benchtop microplate reader that measures protein stability using intrinsic fluorescence – no dyes, no proprietary consumables. From formulation and manufacturing to automation, it delivers reliable melting data with minimal sample prep, helping scientists move faster with confidence and clarity.
Go beyond melting temperature
Tm tells you when proteins unfold. Tagg shows when aggregation begins. Neither tells you if proteins refold.
With MSF, SUPR-DSF measures both melting temperature (Tm) and non-reversibility onset (Tnr), revealing when structural changes become irreversible, often before aggregation is detectable.
Dye-free, in-plate simplicity
SUPR-DSF eliminates extrinsic dyes and extra preparation steps by measuring intrinsic protein fluorescence directly in a single 384-well plate. This reduces hands-on time, lowers error risk, and ensures signals reflect true protein unfolding.
Full-spectrum data you can trust
By capturing the complete intrinsic fluorescence spectrum across the full temperature range, SUPR-DSF reveals subtle stability differences others miss. Rich, high-integrity data enables flexible analysis approaches and confident decision-making, even at therapeutic protein concentrations or in challenging formulations.
Scale stability screening with automation
SUPR-DSF fits seamlessly into automated stability screening workflows, enabling labs to scale condition screening and candidate ranking without adding operational complexity. Its plate-based format supports straightforward integration with liquid handlers for repeatable sample setup and consistent execution across large studies. The result is faster iteration, improved reproducibility, and a stability workflow that can grow with your pipeline.
Designed for cost-efficient screening
Run up to 384 samples in a single experiment using standard, low-cost qPCR plates and seals—no proprietary consumables required. With low 10μL sample volumes and a broad concentration range (from <0.1 mg/mL to >100 mg/mL), SUPR-DSF offers the flexibility to handle diverse protein samples while maintaining seamless integration into existing lab work-flows including automation
Real-world protein applications
SUPR-DSF powers key stages of protein discovery and development, delivering fast, reliable stability and interaction insights that accelerate innovation and reduce experimental bottlenecks.
Protein Engineering & Construct Screening
Rapid Construct Stability
SUPR-DSF ranks hundreds of early protein constructs directly in-plate, revealing thermal stability and potential self-association in hours, enabling faster, confident decisions during protein engineering and discovery programs.
Precision DSF technology
The SUPR-Suite software provides a comprehensive data analysis environment. Users can process emission spectra via barycentric mean, ratio of two wavelengths, or intensity at a single wavelength range.Automatic peak fitting of the first derivative, combined with interactive tools, ensures that DSF data analysis is both straightforward and efficient. Additionally, integrated thermodynamic models allow for precise fitting to determine van ’t Hoff enthalpies and accurate onset temperatures.
Resources
Comprehensive Assessment of Protein Conformational Stability and Unfolding Reversibility in Biotherapeutic Development via Intrinsic DSF and MSF
The development of biotherapeutics requires rigorous evaluation of both conformational and colloidal stability. In this application note, we introduce a new temperature cycling capability for…
Statistical Repeatability of Tm Values Highlights Utility of Protein Stability Screening Automation
Overview To illustrate the accuracy, and repeatability of microplate-based screening with the SUPR-DSF, 16 x 384-well microplates were prepared with lysozyme using a modular approach…
Identification of AAV Serotypes and Characterisation of their Stability by DSF for use in a Gene Therapy Program
Overview In this application note we used the SUPR-DSF to measure the thermal stability of three AAV serotypes: AAV2, AAV5, and AAV6. The SUPR-DSF measured…
Unlock Protein Stability Insights
Gain clear, actionable data on protein folding and interactions—streamlining experiments, saving time, and powering confident decisions throughout your discovery workflow.
