About NEB Tools
NEBtools brings New England Biolabs’ most popular web tools to your iPhone and iPad, allowing you to plan your experiments from anywhere. Use Enzyme Finder to select a restriction enzyme by category, recognition sequence,...
NEBtools brings New England Biolabs’ most popular web tools to your iPhone and iPad, allowing you to plan your experiments from anywhere. Use Enzyme Finder to select a restriction enzyme by category, recognition sequence, overhang or name to find information on that enzyme. Determine buffer and reaction conditions for experiments requiring two restriction enzymes using the Double Digest Finder. When using either of these tools, look for Time-Saver and HF enzymes for the ultimate in convenience. Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to concentration, and calculate basic dilution and molarity. NEB Tools for iOS also enables quick and easy access to NEB Technical Support by phone, email, or web when you need it.
Version History Version 2.6.9 of NEBtools is a minor update that adds Q5-XT Hot Start polymerase to the available selections in Tm Calculator. 2.6.9 May 1 Changes introduced in NEBtools v2.6.7: The restriction enzyme data for Enzyme Finder and Double Digest Finder were updated. NEB Customer Service was revised to Customer Support. 2.6.7 Mar 25 Version 2.6.5 corrects an error in processing the on-board data used by the app which resulted in an incorrect incubation temperature for enzyme SmaI. 2.6.5 09/09/2025 Version 2.6.4 of NEBtools updates th on-board restriction enzyme data set. 2.6.4 08/18/2025 Version 2.6.1 adds support for Q5 Blood Direct 2x Master Mix in Tm Calculator. Revisions were made to the effective monovalent cation concentration associated with some of the buffers used in Tm Calculator. Minor updates were also made to fix bugs. 2.6.1 07/28/2022 Version 2.5.0 updates the restriction enzyme data used by Enzyme Finder and Double Digest. Minor updates were also made to fix reported bugs. 2.5.0 06/14/2022 Version 2.4.0 of NEB Tools updates the on-board restriction enzyme data used by Double Digest and Enzyme Finder. The update also includes the new naming scheme for NEBuffers reflecting the new BSA-free formulations. Enzyme supplement information was also updated. 2.4.0 04/30/2021 This version updates the restriction enzyme data used by Double Digest Finder and Enzyme Finder. It also includes minor updates to protocols and minor correction to the biocalculator modules. This release also addresses data issue that might cause crashes in version 2.3.0. 2.3.1 02/11/2021 This version updates the restriction enzyme data used by Double Digest Finder and Enzyme Finder. It also includes minor updates to protocols and minor correction to the biocalculator modules. 2.3.0 02/05/2021 This version of NEB Tools updates the restriction enzyme data and digest protocols used by Enzyme Finder and Double Digest. Single enzyme digest protocols are easier to obtain from within Double Digest Finder. It is recommended for all existing users. 2.2.0 06/05/2020 NEBtools v2.1.2 fixes several incompatibilty issues with iOS 13 affecting the Double Digest Finder and NEBioCalculator modules. 2.1.2 09/28/2019 This version of NEBtools corrects an issue in the NEBioCalculator module that could lead to apparently incorrect results. The issue resolves automatic changes to the units on certain calculator inputs after the first input value is entered. These automatic changes may be easily overlooked. The changes have been disabled. 2.1.1 08/14/2019 Release v2.1.0 of NEBtools includes updates to the enzyme data, minor feature enhancements to Tm Calculator and NEBioCalculator modules, as well as bug fixes for identified problems that could crash the application under certain conditions. 2.1.0 08/05/2019 NEBtools has been updated to work on both iPhone and iPad running the latest versions of iOS and includes many design enhancements and new functionality. Redesigned Enzyme Finder and Double Digest Finder allow easier enzyme selection. Double Digest Finder includes detailed digestion protocols. NEBtools v2.0.0 also includes several popular calculators from the NEBioCalculator web app. 2.0.0 05/05/2017 Version 1.7.2 of NEB Tools correctly handles number formats in regions using commas in place of decimal points. This allows proper setting of amplicon size in the PCR cycler settings view. 1.7.2 07/26/2013 Corrected US tech support phone number. Upgrades mentioned in v 1.7.0 were maintained. NEB Tools now includes the NEB Tm Calculator for determining optimal annealing temperatures and suggested cycler settings for PCR reactions using NEB polymerases and buffers. The main screen now features easy ways to contact NEB's technical support service. Release 1.7.0 also includes minor updates to the restriction enzyme data used in the Double Digest Finder. 1.7.1 07/24/2013 NEB Tools now includes the NEB Tm Calculator for determining optimal annealing temperatures and suggested cycler settings for PCR reactions using NEB polymerases and buffers. The main screen now features easy ways to contact NEB's technical support service. Release 1.7.0 also includes minor updates to the restriction enzyme data used in the Double Digest Finder. 1.7.0 07/18/2013 This version adds conversions between the old and new NEBuffer systems in Double Digest Finder as an aid to users who need to continue using their stocks of old NEBuffers. 1.6.1 04/07/2013 Updated Enzyme Finder and Double Digest Finder to work with NEB's new and improved restriction enzyme buffers, NEBuffer 1.1, 2.1, 3.1 and CutSmart. 1.6 03/19/2013 Updated enzyme database. Resolved potential error when searching for asymmetric recognition sequences. Updated to work with iPhone 5 and iOS 6. 1.5 12/11/2012 Contains updated restriction enzyme database incorporating new enzymes and buffer changes. New High Fidelity enzymes. RE-Mix Master Mix enzymes. Restored missing R/Y keys in sequence editor. Minor corrections to Double Digest Calculator algorithm. 1.4 07/09/2012 Contains updated restriction enzyme database. Minor bug fixes. Supports iOS 4.3 Last version to support iOS 3.1.3. 1.3.2 08/26/2011 Corrected display of methylated bases in the recognition sequences of EpiMark(TM)-validated enzymes. Tested for compatibility with iOS 4.2. 1.3.1 12/11/2010 Version 1.3.0 is a maintenance release that updates the database and includes a new search category for EpiMark(TM) validated restriction enzymes that are useful in epigenetic analysis. 1.3.0 11/18/2010 Double digestion data has been updated. Compatibility with iOS4 tested. 1.2.0 09/09/2010
Previous Versions
Here you can find the changelog of NEB Tools since it was posted on our website on 2016-09-23 22:06:32.
The latest version is 2.6.9 and it was updated on 2026-06-23 08:44:52. See below the changes in each version.
NEB Tools version 2.6.9
Updated At: 2026-06-02
Changes: Version History Version 2.6.9 of NEBtools is a minor update that adds Q5-XT Hot Start polymerase to the available selections in Tm Calculator. 2.6.9 May 1 Changes introduced in NEBtools v2.6.7: The restriction enzyme data for Enzyme Finder and Double Digest Finder were updated. NEB Customer Service was revised to Customer Support. 2.6.7 Mar 25 Version 2.6.5 corrects an error in processing the on-board data used by the app which resulted in an incorrect incubation temperature for enzyme SmaI. 2.6.5 09/09/2025 Version 2.6.4 of NEBtools updates th on-board restriction enzyme data set. 2.6.4 08/18/2025 Version 2.6.1 adds support for Q5 Blood Direct 2x Master Mix in Tm Calculator. Revisions were made to the effective monovalent cation concentration associated with some of the buffers used in Tm Calculator. Minor updates were also made to fix bugs. 2.6.1 07/28/2022 Version 2.5.0 updates the restriction enzyme data used by Enzyme Finder and Double Digest. Minor updates were also made to fix reported bugs. 2.5.0 06/14/2022 Version 2.4.0 of NEB Tools updates the on-board restriction enzyme data used by Double Digest and Enzyme Finder. The update also includes the new naming scheme for NEBuffers reflecting the new BSA-free formulations. Enzyme supplement information was also updated. 2.4.0 04/30/2021 This version updates the restriction enzyme data used by Double Digest Finder and Enzyme Finder. It also includes minor updates to protocols and minor correction to the biocalculator modules. This release also addresses data issue that might cause crashes in version 2.3.0. 2.3.1 02/11/2021 This version updates the restriction enzyme data used by Double Digest Finder and Enzyme Finder. It also includes minor updates to protocols and minor correction to the biocalculator modules. 2.3.0 02/05/2021 This version of NEB Tools updates the restriction enzyme data and digest protocols used by Enzyme Finder and Double Digest. Single enzyme digest protocols are easier to obtain from within Double Digest Finder. It is recommended for all existing users. 2.2.0 06/05/2020 NEBtools v2.1.2 fixes several incompatibilty issues with iOS 13 affecting the Double Digest Finder and NEBioCalculator modules. 2.1.2 09/28/2019 This version of NEBtools corrects an issue in the NEBioCalculator module that could lead to apparently incorrect results. The issue resolves automatic changes to the units on certain calculator inputs after the first input value is entered. These automatic changes may be easily overlooked. The changes have been disabled. 2.1.1 08/14/2019 Release v2.1.0 of NEBtools includes updates to the enzyme data, minor feature enhancements to Tm Calculator and NEBioCalculator modules, as well as bug fixes for identified problems that could crash the application under certain conditions. 2.1.0 08/05/2019 NEBtools has been updated to work on both iPhone and iPad running the latest versions of iOS and includes many design enhancements and new functionality. Redesigned Enzyme Finder and Double Digest Finder allow easier enzyme selection. Double Digest Finder includes detailed digestion protocols. NEBtools v2.0.0 also includes several popular calculators from the NEBioCalculator web app. 2.0.0 05/05/2017 Version 1.7.2 of NEB Tools correctly handles number formats in regions using commas in place of decimal points. This allows proper setting of amplicon size in the PCR cycler settings view. 1.7.2 07/26/2013 Corrected US tech support phone number.
Upgrades mentioned in v 1.7.0 were maintained. NEB Tools now includes the NEB Tm Calculator for determining optimal annealing temperatures and suggested cycler settings for PCR reactions using NEB polymerases and buffers. The main screen now features easy ways to contact NEB's technical support service. Release 1.7.0 also includes minor updates to the restriction enzyme data used in the Double Digest Finder. 1.7.1 07/24/2013 NEB Tools now includes the NEB Tm Calculator for determining optimal annealing temperatures and suggested cycler settings for PCR reactions using NEB polymerases and buffers. The main screen now features easy ways to contact NEB's technical support service. Release 1.7.0 also includes minor updates to the restriction enzyme data used in the Double Digest Finder. 1.7.0 07/18/2013 This version adds conversions between the old and new NEBuffer systems in Double Digest Finder as an aid to users who need to continue using their stocks of old NEBuffers. 1.6.1 04/07/2013 Updated Enzyme Finder and Double Digest Finder to work with NEB's new and improved restriction enzyme buffers, NEBuffer 1.1, 2.1, 3.1 and CutSmart. 1.6 03/19/2013 Updated enzyme database.
Resolved potential error when searching for asymmetric recognition sequences.
Updated to work with iPhone 5 and iOS 6. 1.5 12/11/2012 Contains updated restriction enzyme database incorporating new enzymes and buffer changes.
New High Fidelity enzymes.
RE-Mix Master Mix enzymes.
Restored missing R/Y keys in sequence editor.
Minor corrections to Double Digest Calculator algorithm. 1.4 07/09/2012 Contains updated restriction enzyme database.
Minor bug fixes.
Supports iOS 4.3
Last version to support iOS 3.1.3. 1.3.2 08/26/2011 Corrected display of methylated bases in the recognition sequences of EpiMark(TM)-validated enzymes.
Tested for compatibility with iOS 4.2. 1.3.1 12/11/2010 Version 1.3.0 is a maintenance release that updates the database and
includes a new search category for EpiMark(TM) validated restriction
enzymes that are useful in epigenetic analysis. 1.3.0 11/18/2010 Double digestion data has been updated.
Compatibility with iOS4 tested. 1.2.0 09/09/2010
NEB Tools version 2.6.5
Updated At: 2025-09-09
Changes: Sep 9, 2025
Version 2.6.5
Version 2.6.5 corrects an error in processing the on-board data used by the app which resulted in an incorrect incubation temperature for enzyme SmaI.
NEB Tools version 2.6.5
Updated At: 2025-09-09
Changes: Version 2.6.5 corrects an error in processing the on-board data used by the app which resulted in an incorrect incubation temperature for enzyme SmaI.
NEB Tools version 2.6.1
Updated At: 2022-07-28
Changes: Jul 28, 2022
Version 2.6.1
Version 2.6.1 adds support for Q5 Blood Direct 2x Master Mix in Tm Calculator. Revisions were made to the effective monovalent cation concentration associated with some of the buffers used in Tm Calculator. Minor updates were also made to fix bugs.
NEB Tools version 1.7.2
Updated At: 2010-01-15
NEB Tools version 2.6.7
Updated At: 1970-01-01
Changes: Changes introduced in NEBtools v2.6.7: The restriction enzyme data for Enzyme Finder and Double Digest Finder were updated. NEB Customer Service was revised to Customer Support.
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