The permeability assay protocol for Caco-2 cells serves as a cornerstone in understanding and predicting the intestinal absorption of pharmaceutical agents.
In a publication in the Journal of Medicinal Chemistry, a collaborative research led by the scientist Dr. H. Day, in conjunction with a consortium of researchers, explored the development of allosteric inhibitors targeting SH2 domain-containing protein tyrosine phosphatase-2 (SHP2) for cancer therapy.
In preclinical research, the choice of the appropriate well system format – whether it's a transwell, non-transwell, or individual transwell – can significantly impact the outcome of experiments. Selecting the correct plate is crucial to working with permeability, toxicity, drug screening, or other assays.
This research, titled Fragment-Based Discovery of a Series of Allosteric-Binding Site Modulators of β‑Glucocerebrosidase, highlights the potential of fragment-based drug discovery (FBDD) and employs permeability assays with CacoReady and PreadyPort plates.
It is well known that food intake changes luminal conditions in the stomach and the small intestine, modifying drug bioavailability. Food-drug interactions are one of the major challenges for oral-administered drugs, even more so if considering the growing use of food supplements and functional foods.
The primary goal of this project is to develop liver-on-chip systems that integrate microfluidics, 3D printing, human-derived liver cells, and liver-derived bioinks.
The article highlights the discovery of a series of azetidine derivatives, known as BGAz compounds, that exhibit potent bactericidal activity against both drug-sensitive Mycobacterium tuberculosis and multidrug-resistant TB strains.
The Blood-Brain Barrier (BBB) is a highly selective barrier that regulates the exchange of substances between the bloodstream and the central nervous system (CNS). It consists of endothelial cells, pericytes, astrocytes, and neurons, forming what is known as the neurovascular unit.
In a study published in the Future Pharmacology Journal, researchers from prestigious French companies examined the impact of four polyols on the permeability of seven active pharmaceutical ingredients (APIs).
