10.5 Exhaustive Release Notes

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Updates New and more powerful computing engine – Data in this engine is built on top of the Eigen library, allowing us to store data more efficiently, and thereby giving data a smaller memory footprint and better performance in computations. Now offering named-user subscription (i.e. username/password) option for authentication through the FlowJo Portal platform. FlowJo is now fully… Read more »

10.5 Release Notes

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New Features: FlowJo Portal Authentication is now available, enabling named-user subscription (i.e. username/password). Includes a new and enhanced computing engine, for improved performance. Drag and drop merging of CSV parameters into data matrices now supported. FlowJo is now fully GDPR compliant. Warnings: When saving an ACS file from a FlowJo Envoy based workspace, the default location prompt… Read more »

CSV Parameter Merging

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Within FlowJo v10.5+ new parameters saved in CSV format can be merged with existing data matrices by dragging and dropping directly onto appropriately matched data files. How To Mergeable CSV files must meet certain criteria in order to merge correctly with the target data file: The CSV to merge must have the same number of… Read more »

FlowJo Portal Licensing

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FlowJo v10.5+ offers a named-user subscription (i.e. username/password) option for authentication through the FlowJo Portal platform. This latest licensing tool means researchers no longer need to worry about losing or breaking their FlowJo Dongle, and their license won’t be bound to a particular computer in order to analyze data in FlowJo. For detailed information regarding the… Read more »

10.4.2 Exhaustive Release Notes

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Updates User interface on HDPI displays now correctly sized at install time. Fixed incorrect statistics generated from certain MQD data files. Fixed issue of Downsample populations being dropped from ACS files. Corrected $PnE keyword value changes occurring during export. FACSLyric data now utilizes standard BD transformations by default. Known Issues Authentication 24-hour offline grace period… Read more »

10.4.2 Release Notes

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New Features FlowJo icons on high resolution monitors now correctly sized. FACSLyric data now gets its own badge in FlowJo’s Cytometers preferences, and utilizes standard BD transformations by default. Bugs Fixed Fixed incorrect statistics reported for some MQD data files. Downsample populations no longer dropped from ACS files. $PnE keyword value no longer altered during export…. Read more »

Spillover Spreading Matrix

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The Spillover Spreading Matrix (SSM) is a measure of the performance of an instrument for a given panel.  It reflects the Poisson-based error in fluorescence quantification.  SSM’s can be calculated for a set of single stain controls in the Compensation Editor. This tool works for matrices created in FlowJo, as the single stain controls are… Read more »

10.4.1 Exhaustive Release Notes

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Updates Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Stopped Compensation Wizard matrix re-calculations (ad infinitum). Workspace Title will no longer automatically revert to “unsaved” immediately after a save. Special considerations for Cytek data scaling implemented. Color Mapping axis keyboard shortcuts are now functional…. Read more »

10.4.1 Release Notes

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New Features Search bar added to parameter selectors in the graph window. Color mapped (aka “heat map statistic”) parameter labeling in layouts. Bugs Fixed Proliferation Platform Improvements Stopped Compensation Wizard matrix re-calculations (ad infinitum). More Info For more details on features in this release, and a comprehensive list of known issues visit the exhaustive release notes…. Read more »

Color Mapping

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Color mapping in FlowJo’s graph window allows users to visualize a third parameter in the two-dimensional display, by illustrating a Median value for any tertiary parameter in a color scale applied to the dots displayed. Accessing the Color Map Checking the box for “Color Axis” will display the expression levels of a third parameter represented… Read more »