This page contains suggestions for improving the fit of difficult to model cytometric data. Preprocessing Removing doublets, dead cells, and debris will improve the condition of data. Whatever steps you typically follow to do this can be applied to cell cycle data.  Some common methods for doing this are: Doublets can be removed by plotting… Read more »

There are four controllers for the 3D Viewer: Plot, Top Bar Controls, Parameters and Color   Plot From the Plot, set the parameters for X, Y and Z axes and assign a color if wanted. Setting Size: event size will scale based on how positive/negative events are for a specified parameter. More positive events will… Read more »

Welcome to FlowJo, a software application with an integrated environment for viewing and analyzing flow cytometric data. The environment is presented as the Workspace, which contains a list of loaded samples (experimental data), statistics, gates, and other analyses, as well as tabular and graphical layouts. The workspace is saved as a FlowJo document on your hard… Read more »