Join Pedro, the author of the groundbreaking study on the scalable manufacturing of gene-modified hMSCs, as he delves deep into the world of adherent cell manufacturing to microcarriers…
Counting cells with microcarriers
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NucleoCounter® NC-3000™
Advanced Image Cytometer
- A variety of fixed and build-your-own assays
- High-speed cell counting and advanced data analysis
- No warm-up or calibration required
- No liquid waste produced
- 21 CFR Part 11/GMP-ready software
The NucleoCounter® NC-3000™ advanced image cytometer utilizes fluorescence imaging to characterize cell properties. The instrument performs high-speed plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute Cell Cycle Assay, a selection of Apoptosis Assays and the standard Cell Count and Viability Assay.
With the FlexiCyte™ license, you can create protocols with options for light source, emission filters and other detailed settings. The accompanying NucleoView™ software is ideal for intuitive data analysis and has unlimited licenses. You can view data in plot graphs and interlinked images, which can be saved for later on-demand analysis.
This advanced image cytometer ensures flow-like analysis with high reproducibility, operates with low sample volumes, and is calibration-free.
Used with the disposable Via1-Cassette™, this closed cell counting system helps you stay protected when handling samples. No pipettes, no exposure to (toxic) reagents, and minimal clean-up make the NucleoCounter® NC-3000™ a safe option.
Read more about the Cassette technology
- Assays: 5-minute cell cycle analysis, 1-minute apoptosis assay, 1-minute GFP assay
- Exports to data analysis tools, such as FCS Express and FlowJo
- PDF reports
- Unlimited software installs and free updates
- 5-channel open platform
| Application type(s) | Fixed assays: Cell count & viability, Cell cycle, Mitochondrial potential, Vitality, Caspase, Annexin V, Transfection efficiency, and DNA fragmentation assays. User-adapted protocols with the FlexiCyte™ module |
| Cell type(s) | Mammalian, yeast, insect, avian |
| Software | NucleoView™ |
| Consumable(s) | Via1-Cassette™, NC-Slide A2™ and NC-Slide A8™ |
| Capacity (Analysis time) | Variable, depending on consumable and assay type |
| Loading volume | 60 μl (Via1-Cassette™), 30 μl (NC-Slide A2™), 10 μl (NC-Slide A8™) |
| Analysis volume | 3.2 μl sample (Via1-Cassette™), 0.8 – 16 μl sample (NC-Slide A2™, NC-Slide A8™) |
| Optimal range | 5×104 to 5×106 cells/ml |
| Staining dye(s) | Hoechst 33342, JC-1, VitaBright-43™, VitaBright-48™, Acridine orange (AO), DAPI, Propidium iodide (PI), User-defined |
| Excitation | Seven LED light sources with peaks at 365 nm, 405 nm, 475 nm, 530 nm, 630 nm + 2 white LEDs |
| Emission (nm) | Nine interchangeable emission filters: 430/20, 470/55, 475/15, 530/15, 560/35, 580/25, 675/75, 630 LP, 740/60 |
| Optics | Lens with x2 magnification, 1/2″ CCD camera with 1392×1040 pixels |
| Data output | Images, tables, histograms, scatter plots |
| Data export formats | .cm, .csv, .pdf, .cmpp, .bmp, .tiff, .acs, .fcs |
| Product Number |
970-3002 (Fixed Analytical Assays); 970-3003 (FlexiCyte™) |
| Size |
29 x 29 x 31 cm (W x H x D) |
| Weight |
14 kg (30.9 lb) |
| Supply voltage |
24VDC (100–240 V ~ 50–60 Hz) |
Sampling Devices, Reagents & Test Kits for the NucleoCounter® NC-3000™ Advanced Cell Analyzer.
| Title | Categories | Download |
|---|---|---|
| A comparison of DNA quantitation by image and flow cytometry | Scientific Poster | |
| A decrease in cellular thiols caused by drug-induced | Scientific Poster | |
| A novel rapid apoptosis assay based on thiol redox status | Scientific Poster | |
| A Novel Scalable Production Platform for Lentiviral Vectors Based on Human Suspension Cell Lines | Scientific Poster | |
| A simple and rapid method for DNA content measurements | Scientific Poster | |
| App Note Annexin V Assay NC-3000™ | Application Note | |
| App Note Caspase 3, 7, 8/9 (FLICA) Assay NC-3000™ | Application Note | |
| App Note Cell Count & Viability Aggregated Cells Via1-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count Aggregated Cells NC-Slides NC-3000™ | Application Note | |
| App Note Cell Count Aggregated Cells NC-Slides™ Reagent A100 and B NC-3000™ | Application Note | |
| App Note Cell Count Aggregated Cells Via1-Cassette™ Reagent A100 and B NC-3000™ | Application Note | |
| App Note Cell Count and Viability Aggregated Cells NC-Slide A2™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Aggregated Cells Via1-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Insect Cells NC-Slides A8™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Insect Cells Via1-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Mammalian Cells NC-slides A2™ Reagent A100 and B NC-3000™ | Application Note | |
| App Note Cell Count and Viability Mammalian Cells NC-Slides A8™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Mammalian Cells NC-Slides Method 2 NC-3000™ | Application Note | |
| App Note Cell Count and Viability Mammalian Cells PI-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Mammalian Cells Via1-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Mammalian Cells Via1-Cassette™ Reagent A100 and B NC-3000™ | Application Note | |
| App Note Cell Count and Viability Purified Leukocyte NC-3000™ | Application Note | |
| App Note Cell Count and Viability Via1-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Whole Blood NC-Slides™ NC-3000™ | Application Note | |
| App Note Cell Count and Viability Yeast Cells PI-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count Mammalian Cells PI-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Count Yeast Cells PI-Cassette™ NC-3000™ | Application Note | |
| App Note Cell Cycle Fixed Cells NC-3000™ | Application Note | |
| App Note DNA Fragmentation NC-3000™ | Application Note | |
| App Note FlexiCyte™ Assay NC-3000™ | Application Note | |
| App Note GFP Transfection Hoechst and PI-Cassette™ NC-3000™ | Application Note | |
| App Note Mitochondrial Potential NC-3000™ | Application Note | |
| App Note Two Step Cell Cycle NC-3000™ | Application Note | |
| App Note Vitality Assay NC-3000™ | Application Note | |
| Cell count determination of aggregated HEK293 suspension cells and Vero cells on microcarriers | Scientific Poster | |
| Delineating the Critical Process Parameters for Cell Therapy Cryopreservation | Scientific Poster | |
| NC-3000™ Product Video | Video | |
| NucleoCounter® NC-3000™ Brochure | Brochure | |
| NucleoCounter® NC-3000™ Validation Plan | Brochure | |
| Quantitative assesment of apoptotic events using image and flow cytometry | Scientific Poster | |
| Quick Guide: NucleoCounter® NC-3000™ | User Guide | |
| Software User Guide: NucleoView™ NC-3000™ | User Guide | |
| Tech Note: Cleaning Guide for NucleoCounter® NC-3000™ | Technical Note | |
| Tech Note: LED Life Time Consideration for NucleoCounter® NC-3000™ | Technical Note | |
| Tech Note: Variations and Statistics for NucleoCounter® NC-3000™ | Technical Note | |
| The Effect of Cryomedia Selection and Transient Warming Events on Post-Cryopreservation Human MSC Function | Scientific Poster | |
| Transition to GMP, chemically-defined, xeno-free cryopreservation media increases post-thaw functionality of clinically relevant cell banks | Scientific Poster | |
| User Guide: NucleoCounter® NC-3000™ Advanced Image Cytometer | User Guide | |
| Whitepaper: 21 CFR Part 11 Compatible Software for NucleoCounter® NC-3000™ | Whitepaper |
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