Skip to main content
Log in

Identifying the presence of bacteria on digital images by using asymmetric distribution with k-means clustering algorithm

  • Published:
Multidimensional Systems and Signal Processing Aims and scope Submit manuscript

Abstract

This paper is mainly aimed at the decomposition of image quality assessment study by using Three Parameter Logistic Mixture Model and k-means clustering (TPLMM-k). This method is mainly used for the analysis of various images which were related to several real time applications and for medical disease detection and diagnosis with the help of the digital images which were generated by digital microscopic camera. Several algorithms and distribution models had been developed and proposed for the segmentation of the images. Among several methods developed and proposed, the Gaussian Mixture Model (GMM) was one of the highly used models. One can say that almost the GMM was playing the key role in most of the image segmentation research works so far noticed in the literature. The main drawback with the distribution model was that this GMM model will be best fitted with a kind of data in the dataset. To overcome this problem, the TPLMM-k algorithm is proposed. The image decomposition process used in the proposed algorithm had been analyzed and its performance was analyzed with the help of various performance metrics like the Variance of Information (VOI), Global Consistency Error (GCE) and Probabilistic Rand Index (PRI). According to the results, it is shown that the proposed algorithm achieves the better performance when compared with the previous results of the previous techniques. In addition, the decomposition of the images had been improved in the proposed algorithm.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+
from €37.37 /Month
  • Starting from 10 chapters or articles per month
  • Access and download chapters and articles from more than 300k books and 2,500 journals
  • Cancel anytime
View plans

Buy Now

Price includes VAT (Netherlands)

Instant access to the full article PDF.

Fig. 1
The alternative text for this image may have been generated using AI.
Fig. 2
The alternative text for this image may have been generated using AI.
Fig. 3
The alternative text for this image may have been generated using AI.
Fig. 4
The alternative text for this image may have been generated using AI.
Fig. 5
The alternative text for this image may have been generated using AI.
Fig. 6
The alternative text for this image may have been generated using AI.
Fig. 7
The alternative text for this image may have been generated using AI.
Fig. 8
The alternative text for this image may have been generated using AI.
Fig. 9
The alternative text for this image may have been generated using AI.
Fig. 10
The alternative text for this image may have been generated using AI.
Fig. 11
The alternative text for this image may have been generated using AI.
Fig. 12
The alternative text for this image may have been generated using AI.
Fig. 13
The alternative text for this image may have been generated using AI.
Fig. 14
The alternative text for this image may have been generated using AI.

Similar content being viewed by others

References

  • Akhavan, R., & Faez, K. (2014). A novel retinal blood vessel segmentation algorithm using fuzzy segmentation. International Journal of Electrical and Computer Engineering, 4(4), 561–572.

    Google Scholar 

  • Bechar, M. E., Settouti, N., Barra, V., et al. (2018). Semi-supervised superpixel classification for medical images segmentation: Application to detection of glaucoma disease. Multidimensional Systems and Signal Processing, 29, 979–998.

    Article  MathSciNet  Google Scholar 

  • J. Bilmes, A. Vahdat, W. Hsu, E.-J. Im, (1997). Empirical observations of probabilistic heuristics for the clustering problem. Technical Report ICSI-TR097–018, ICSI.

  • Cimpoi, M., Maji, S., Kokkinos, I., & A. Vedaldi A. . (2016). Deep filter banks for texture recognition, description, and segmentation. International Journal of Computer Vision, 118(1), 65–94.

    Article  MathSciNet  Google Scholar 

  • Dias, P. A., Dunkel, T., Fajado, D. A. S., et al. (2016). Image processing for identification and quantification of filamentous bacteria in in situ acquired images. BioMedical Engineering OnLine, 15(64), 1–21.

    Google Scholar 

  • Guang, W. X., & Chen, S. H. (2012). An improved image segmentation algorithm based on two-dimensional otsu method. Information Science Letters, 1(2), 77–83.

    Article  Google Scholar 

  • Hay, E. A., & Parthasarathy, R. (2018). Performance of convolutional neural networks for identification of bacteria in 3D microscopy datasets. PLoS Computational Biology, 14(12), 1–24.

    Article  Google Scholar 

  • Jeckel, H., & Drescher, K. (2021). Advances and opportunities in image analysis of bacterial cells and communities. FEMS Microbiology Reviews. https://doi.org/10.1093/femsre/fuaa062

    Article  Google Scholar 

  • Jyothirmayi, T., Srinivasa Rao, K., & Srinivasa Rao RaoSatyanarayana Ch, PCh. (2016). Image segmentation based on doubly truncated generalized laplace mixture model and k means clustering. International Journal of Electrical and Computer Engineering, 6(5), 2188–2196.

    Google Scholar 

  • Khamparia, A., Bharati, S., Podder, P., P, et al. (2021). Diagnosis of breast cancer based on modern mammography using hybrid transfer learning. Multidimensional Systems and Signal Processing, 32, 747–765. https://doi.org/10.1007/s11045-020-00756-7

    Article  MATH  Google Scholar 

  • Lee, D., Lee, J., Ko, J., Yoon, J., Ryu, K., & Nam, Yo. (2019). Deep learning in MR image processing. Investigate Magnetic Resonance Imaging, 23(2), 81–99.

    Article  Google Scholar 

  • Li, Z., Liu, M., Wang, H., Yang, Y., Chen, J., & Jin, G. (2013). Gray-scale edge detection and image segmentation algorithm based on mean shift. Indonesian Journal of Electrical Engineering and Computer Science, 11(3), 1414–1421.

    Google Scholar 

  • Liang, Y., Sun, L., Ser, W., et al. (2017). Hybrid threshold optimization between global image and local regions in image segmentation for melasma severity assessment. Multidimensional Systems and Signal Processing, 28, 977–994.

    Article  Google Scholar 

  • Lie, T., & Sewehand, W. (1992). Statistical approach to X -ray CT imaging and its applications in image analysis. IEEE Transactions on Medical Imaging, 11(1), 53–61.

    Article  Google Scholar 

  • Lie, T., & Udupa, J. K. (1993). Performance evaluation of finite normal mixture model based image segmentation. IEEE Transactions on Image Processing, 12(10), 1153–1169.

    Google Scholar 

  • McLachlan, G. J., & Krishnan, T. (1997). The EM Algorithm and Extensions. Wiley.

    MATH  Google Scholar 

  • B. A. Mohamed, H. M. Afify, (2018). Automated classification of bacterial images extracted from digital microscope via bag of words model. 9th Cairo International Biomedical Engineering Conference (CIBEC), Cairo, Egypt, 86–89.

  • Nahar, M., & Ali, M. S. (2014). An improved approach for digital image edge detection. International Journal of Recent Development in Engineering and Technology, 2(3), 14–20.

    Google Scholar 

  • Preetha, V., & Pandi Selvi, P. (2018). Identification of bacteria using digital image processing. International Journal of Engineering Research in Computer Science and Engineering, 5(3), 254–258.

    Google Scholar 

  • Sharma, A., Kumar, S., & Singh, S. N. (2019). Brain tumor segmentation using DE embedded OTSU method and neural network. Multidimensional Systems and Signal Processing, 30, 1263–1291.

    Article  MathSciNet  Google Scholar 

  • Srinivas, Y., & Srinivas Rao, K. (2007). Unsupervised image segmentation using finite doubly truncated Gaussian mixture model and hierarchical clustering. Journal of Current Science, 93(4), 507–514.

    Google Scholar 

  • Tatusov, R. L., Koonin, E. V., & Lipman, D. J. (1997). A genomic perspective on protein families. Science, 278(5338), 631–637. https://doi.org/10.1126/science.278.5338.631 PMID: 9381173.

    Article  Google Scholar 

  • Unnikrishnan, R., Pantofaru, C., & Hebert, M. (2007). Toward objective evaluation of image segmentation algorithms. IEEE Transactions on Pattern Analysis and Machine Intelligence, 29(6), 929–944.

    Article  Google Scholar 

  • M. F. Wahid, T. Ahmed, M. A. Habib, (2018). Classification of microscopic images of bacteria using deep convolutional neural network. 10th International Conference on Electrical and Computer Engineering (ICECE), Dhaka, Bangladesh, 217–220.

  • Wang, H., Kodmir, H. C., Qiu, Y., et al. (2020). Early detection and classification of live bacteria using time-lapse coherent imaging and deep learning. Light Science Applications, 9(118), 1–17.

    Google Scholar 

  • Xin, J., Wang, Z., Z., S. Tian, , et al. (2017). NMR image segmentation based on unsupervised extreme learning machine. Multidimensional Systems and Signal Processing, 28, 1013–1030.

    Article  Google Scholar 

  • Yin, Y., & Ding, Y. (2009). A close to real-time prediction method of total coliform bacteria in foods based on image identification technology and artificial neural network. Food Research International, 42(1), 191–199. https://doi.org/10.1016/j.foodres.2008.10.006

    Article  Google Scholar 

  • Zhang, Y., Jiang, H., Ye, T., & Juhas, M. (2021). Deep learning for imaging and detection of microorganisms. Trends in Microbiology, 29(7), 569–572.

    Article  Google Scholar 

  • Zhang, Z. H., Chen, C., Sun, J., & Chan, K. L. (2003). EM algorithms for gaussian mixtures with split-and-merge operation. Pattern Recognition, 36(9), 1973–1983.

    Article  Google Scholar 

  • Zhong, Z., Wang, M., Gao, W., et al. (2021). A novel multisource pig-body multifeature fusion method based on gabor features. Multidimensional Systems and Signal Processing, 32, 381–404. https://doi.org/10.1007/s11045-020-00744-x

    Article  MATH  Google Scholar 

  • Zieliński, B., Plichta, A., Misztal, K., Spurek, P., Brzychczy-Włoch, M., & Ochońska, D. (2017). Deep learning approach to bacterial colony classification. PLoS ONE, 12(9), 1–18.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Yu-Chen Hu.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Satyanarayana, K.V., Rao, N.T., Bhattacharyya, D. et al. Identifying the presence of bacteria on digital images by using asymmetric distribution with k-means clustering algorithm. Multidim Syst Sign Process 33, 301–326 (2022). https://doi.org/10.1007/s11045-021-00800-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Version of record:

  • Issue date:

  • DOI: https://doi.org/10.1007/s11045-021-00800-0

Keywords

Profiles

  1. Yu-Chen Hu