Lipid droplets have key roles in energy storage and lipid metabolism. This Review discusses tools used for assessing lipid droplet heterogeneity, and how their heterogeneous composition and interactions with other organelles influence physiology and contribute to disease.

This Review discusses new insights into the intricate regulation of mitochondrial protein synthesis, the pathological consequences of its dysfunction in cancer and immunity, and the complexity of therapeutic targeting of mitochondrial translation.

SPAC-seq combines spatial transcriptomics and CRISPR screening within the tissue context, to identify how gene perturbations influence cell states and tissues.

Despite advances in protein structure prediction, how proteins fold remains unclear. Understanding protein (mis)folding will require single-molecule techniques and advanced computation tools.

In this Tools of the Trade, Chen & Cheng (Barna lab) describe the development of Ribo-Tweezer, a method that enables the targeted degradation of individual ribosomal proteins of the cytoplasmic ribosome.

This study shows that neutral lipids regulate endoplasmic reticulum (ER) membrane curvature and bendability during ER-phagy, and finds that a COPII protein helps couple ER cargo to the autophagy receptor.