We provide raw material reagents and analytical services for quality control of biological drugs and other process-related impurity testing.

Molecular Level Analysis for Biological Drugs

DNA Barcoding

INQUIRY

Introduction

DNA barcoding is a molecular technique with the principle of using 400-800 bp long standardized unique DNA sequences to differentiate and assign taxonomy to species. DNA barcoding allows for fast, reliable, automatable and cost-effective species identification by users with little or no taxonomic experience. Identifications are usually made by comparing unknown sequences against known species DNA barcodes via alignment searching (BLAST) or distance-based tree construction.

Fig.1 DNA Barcoding Fig.1 The procedures for DNA barcoding

Applications

Taxonomy and Biodiversity Studies
Species identification, species richness and distribution assessment
Food Authentication
To analyze the species composition of food commodities or track adulterations in food products for quality assurance, quality control, and food safety
Forensic Investigations
To analyze samples from alleged wildlife crime incidents or identify plant materials found at crime scenes
Agriculture and Crop Science
To assist in identifying crop varieties and their wild relatives, facilitating breeding programs and genetic resource management

Our Featured Services

Creative Diagnostics provides standard species identification services based on DNA barcoding technique, as well as enhanced phylogenetic tree construction services. Whether your samples are from animals or plants, we provide valuable genetic markers to support your research.

Sample Requirements for Analysis
Fresh animal or plant tissues, or extracted genomic DNA samples (genomic DNA concentration should be no less than 20 ng/μL, with a total volume of at least 20 μL), and shipped to us at low temperature

Available DNA Barcoding Genes

Species	DNA Barcoding
Animals Mitochondrial DNA (mtDNA)	Mitochondrial cytochrome c oxidase subunit I gene (COI, COX1, CO1)
	16S rRNA
	12S rRNA
Plants Chloroplast DNA (cpDNA)	Ribulose bisphosphate carboxylase large subunit gene (rbcL)
	Maturase K (matK)
	tRNA gene for leucine (trnL)
	A non-coding region between tRNA gene for histidine (trnH) and photosystem II complex (psbA) genes (trnH-psbA)

Deliverables of Standard Species Identification Service
Project report (including PCR product electropherogram, sequencing results, data analysis and species identification reference results)

Online Inquiry