{"id":31876,"date":"2021-10-28T14:12:10","date_gmt":"2021-10-28T12:12:10","guid":{"rendered":"https:\/\/phiab.com\/?p=31876"},"modified":"2023-11-01T11:07:15","modified_gmt":"2023-11-01T10:07:15","slug":"holomonitor-general-capture-protocol","status":"publish","type":"post","link":"https:\/\/phiab.com\/protocol\/general-capture\/","title":{"rendered":"General Capture Assay"},"content":{"rendered":"<div class=\"wp-block-group is-style-banner-image is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\"><figure class=\"wp-block-image size-full background-image is-landscape block-editor-block-list__block block-editor-block-list__first\" style=\"--phi-media-width:1536px;--phi-media-height:512px;--phi-aspect-ratio:3;\">\n    <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt class=\"wp-image-26509 lazyload figure__media\" data-media-id=\"26509\" width=\"1536\" height=\"512\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner-300x100.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner-1024x341.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner-768x256.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner-150x50.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner-512x171.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner-1280x427.jpg 1280w\" data-sizes=\"auto\">\n  <\/figure>\n  <div class=\"wp-block-columns alignfull is-style-no-collapse is-layout-flex wp-container-core-columns-layout-1 wp-block-columns-is-layout-flex block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\"><div class=\"wp-block-column is-vertically-aligned-bottom is-style-default is-layout-flow wp-block-column-is-layout-flow block-editor-block-list__layout is-empty block-editor-block-list__block block-editor-block-list__first\" style=\"flex-basis:15%;\"><\/div>\n    <div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second\" style=\"flex-basis:70%;\">\n      <h1 class=\"wp-block-heading has-text-align-center is-style-default has-white-color has-transparent-black-background-color has-text-color has-background has-huge-font-size block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" id=\"h-general-capture-assay\" style=\"font-weight:600;\">General Capture Assay<\/h1>\n    <\/div>\n    <div class=\"wp-block-column is-vertically-aligned-center is-layout-flow wp-block-column-is-layout-flow block-editor-block-list__layout is-empty block-editor-block-list__block block-editor-block-list__last\" style=\"flex-basis:15%;\"><\/div>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-center floated-parent\" style=\"grid-template-columns:auto 20%;--phi-media-width:300px;--phi-media-height:300px;--phi-aspect-ratio:1;--phi-media-max-width:20%;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n    <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"general-capture-icon\" class=\"wp-image-31885 size-full lazyload figure__media\" data-media-id=\"31885\" width=\"300\" height=\"300\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/general-capture-icon.png\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/general-capture-icon.png 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/general-capture-icon-150x150.png 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/general-capture-icon-100x100.png 100w\" data-sizes=\"auto\">\n  <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-highlight wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">This protocol helps to set up a <strong>General Capture Assay<\/strong> using<a href=\"https:\/\/phiab.com\/holomonitor\/\"> HoloMonitor\u00ae M4<\/a> and the HoloMonitor\u00ae. <a href=\"https:\/\/phiab.com\/holomonitor\/cell-imaging-software\/\">App Suite software<\/a>. The HoloMonitor\u00ae General Capture Assay provides time-lapse images for further analysis by any other <a href=\"https:\/\/phiab.com\/holomonitor\/live-cell-assays\">HoloMonitor\u00ae App Suite assays<\/a>.<\/p>\n    <div class=\"wp-block-buttons alignwide is-horizontal is-content-justification-center is-layout-flex wp-container-core-buttons-layout-1 wp-block-buttons-is-layout-flex block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\">\n      <div class=\"wp-block-button\">\n        <a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/phiab.com\/reports\/AppSuite-4.0\/AppSuite-protocol-general-capture.pdf\">Download this protocol<\/a>\n      <\/div>\n    <\/div>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-group is-style-curve is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <div class=\"wp-block-columns alignwide is-layout-flex wp-container-core-columns-layout-2 wp-block-columns-is-layout-flex block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\"><div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__first\"><h2 class=\"wp-block-heading has-phi-blue-color has-text-color block-editor-block-list__block block-editor-block-list__first\" id=\"requirements\">Requirements:<\/h2>\n      <ul class=\"has-normal-font-size wp-block-list block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\"><li class=\"block-editor-block-list__block block-editor-block-list__first\"><a href=\"https:\/\/phiab.com\/holomonitor\/cell-imaging-system\/\">HoloMonitor\u00ae M4<\/a>, placed in incubator<\/li>\n        <li class=\"block-editor-block-list__block block-editor-block-list__second\">\n          <a href=\"https:\/\/phiab.com\/holomonitor\/cell-imaging-software\/\">HoloMonitor\u00ae M4&nbsp;App Suite<\/a>\n        <\/li>\n        <li class=\"block-editor-block-list__block\"><strong>Culture vessel<\/strong> of choice with cells<\/li>\n        <li class=\"block-editor-block-list__block\"><a href=\"https:\/\/phiab.com\/holomonitor\/hololids\/?swcfpc=1\">HoloLid\u2122 <\/a>for selected vessel<\/li>\n        <li class=\"block-editor-block-list__block block-editor-block-list__last\"><strong>Vessel holder<\/strong> for selected vessel<\/li>\n      <\/ul>\n    <\/div>\n    <div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second\"><h2 class=\"wp-block-heading has-phi-blue-color has-text-color block-editor-block-list__block block-editor-block-list__first\" id=\"output\">Output:<\/h2>\n      <ul class=\"wp-block-list block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\">\n        <li class=\"block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\">\n          <strong>Selected data analysis depended<\/strong>\n        <\/li>\n      <\/ul>\n    <\/div>\n    <div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__last\"><h2 class=\"wp-block-heading has-phi-blue-color has-text-color block-editor-block-list__block block-editor-block-list__first\" id=\"reanalysis\">Reanalysis:<\/h2>\n      <ul class=\"is-style-default wp-block-list block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second\">\n        <li class=\"block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\">Guided assays<ul class=\"wp-block-list block-editor-block-list__layout\"><li class=\"block-editor-block-list__block block-editor-block-list__first\">\n              <a href=\"https:\/\/phiab.com\/holomonitor\/cell-qc-assay\/?swcfpc=1\">Cell Quality Control<\/a>\n            <\/li>\n            <li class=\"block-editor-block-list__block block-editor-block-list__second\">\n              <a href=\"https:\/\/phiab.com\/holomonitor\/cell-proliferation-assay\/\">Kinetic Cell Proliferation<\/a>\n            <\/li>\n            <li class=\"block-editor-block-list__block\">\n              <a href=\"https:\/\/phiab.com\/applications\/kinetic-motility\/?swcfpc=1\">Kinetic Cell Motility<\/a>\n            <\/li>\n            <li class=\"block-editor-block-list__block block-editor-block-list__last\">\n              <a href=\"https:\/\/phiab.com\/applications\/dose-response\/?swcfpc=1\">Kinetic Dose Response<\/a>\n            <\/li>\n          <\/ul>\n        <\/li>\n      <\/ul>\n      <ul class=\"wp-block-list block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__last\">\n        <li class=\"block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\">In-depth analysis<ul class=\"wp-block-list block-editor-block-list__layout\"><li class=\"block-editor-block-list__block block-editor-block-list__first\">\n              <a href=\"https:\/\/phiab.com\/applications\/single-cell-tracking\/?swcfpc=1\">Single Cell Tracking<\/a>\n            <\/li>\n            <li class=\"block-editor-block-list__block block-editor-block-list__second\">\n              <a href=\"https:\/\/phiab.com\/holomonitor\/cell-morphology-assay\/?swcfpc=1\">Cell Morphology<\/a>\n            <\/li>\n            <li class=\"block-editor-block-list__block block-editor-block-list__last\">\n              <a href=\"https:\/\/phiab.com\/holomonitor\/wound-healing-assay\">Wound Healing<\/a>\n            <\/li>\n          <\/ul>\n        <\/li>\n      <\/ul>\n    <\/div>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-group alignwide is-style-curve has-phi-blue-background-color has-background is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <h2 class=\"wp-block-heading alignwide is-style-page-break has-large-font-size block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" id=\"h-preparations\">\n    <strong>PREPARATIONS<\/strong>\n  <\/h2>\n<\/div>\n\n<h3 class=\"wp-block-heading alignwide has-small-large-font-size\" id=\"h-materials\">Materials:<\/h3>\n\n<div class=\"wp-block-group alignwide is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <ul class=\"wp-block-list block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\"><li class=\"block-editor-block-list__block block-editor-block-list__first\"><strong><a href=\"https:\/\/phiab.com\/holomonitor\/?swcfpc=1\">HoloMonitor\u00ae M4<\/a><\/strong>, placed inside the incubator<\/li>\n    <li class=\"block-editor-block-list__block block-editor-block-list__second\">HoloMonitor\u00ae <a href=\"https:\/\/phiab.com\/holomonitor\/cell-imaging-software\/?swcfpc=1\">App Suite software<\/a><\/li>\n    <li class=\"block-editor-block-list__block\"><strong>Cell culture vessel<\/strong>. Please check <a href=\"https:\/\/phiab.com\/protocol\/recommended-vessel-list\/\">our list<\/a> of recommended vessels.<\/li>\n    <li class=\"block-editor-block-list__block\"><a href=\"https:\/\/phiab.com\/holomonitor\/hololids\/?swcfpc=1\">HoloLid\u2122<\/a> for the selected vessel<\/li>\n    <li class=\"block-editor-block-list__block\"><strong>Vessel holder<\/strong> for selected vessel<\/li>\n    <li class=\"block-editor-block-list__block block-editor-block-list__last\"><a href=\"https:\/\/phiab.com\/resources\/manuals-protocols\/\">Setup and Operation Manual<\/a> for HoloMonitor\u00ae<sub><sup> <\/sup><\/sub>M4<\/li>\n  <\/ul>\n<\/div>\n\n<h2 class=\"wp-block-heading alignwide has-small-large-font-size\" id=\"h-steps\">Steps<\/h2>\n\n<div class=\"wp-block-group alignwide is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\"><div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-top is-style-border is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__first\" style=\"--phi-media-width:708px;--phi-media-height:474px;--phi-aspect-ratio:1.4936708860759;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"calibration\" class=\"wp-image-31309 size-full lazyload figure__media\" data-media-id=\"31309\" width=\"708\" height=\"474\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/calibration.png\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/calibration.png 708w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/calibration-300x201.png 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/calibration-150x100.png 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/calibration-512x343.png 512w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/calibration.png\">\n      <\/div>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">Seed the cells with about <strong>5&nbsp;% confluence<\/strong> (ca. 6000 \u2013 11000&nbsp;cells\/cm<sup>2<\/sup>).<\/p>\n      <p class=\"is-style-indented wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">\u25baPlease note that too few cells may lead to inadequate results due to auto-focus failure.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block\">Place the vessel in the incubator and let <strong>cells attach<\/strong> for 2-24&nbsp;hours.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block\"><strong>Start <\/strong>the <strong>software <\/strong>and wait for complete instrument <strong>initialization<\/strong>.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">Run an <strong>auto-calibration<\/strong>. With successful calibration (as shown in the picture), the instrument is ready to use.<\/p>\n    <\/div>\n  <\/div>\n  <figure class=\"wp-block-table alignwide is-style-stripes block-editor-block-list__block block-editor-block-list__second\">\n    <table>\n      <tbody><tr><td>\n            <strong>Vessel<\/strong>\n          <\/td><td class=\"has-text-align-center\" data-align=\"center\">\n            <strong>Vendor cat.<br>number<\/strong>\n          <\/td><td class=\"has-text-align-center\" data-align=\"center\">\n            <strong>HoloLid\u2122<\/strong>\n          <\/td><td class=\"has-text-align-center\" data-align=\"center\">\n            <strong>Final working<br>volume<\/strong>\n          <\/td><td class=\"has-text-align-center\" data-align=\"center\">\n            <strong>Growth area,<br>cm2\/well<\/strong>\n          <\/td><td class=\"has-text-align-center\" data-align=\"center\">\n            <strong>Vessel cut in<br>a holder<\/strong>\n          <\/td>\n        <\/tr><tr><td>Sarstedt TC-dish 35<\/td><td class=\"has-text-align-center\" data-align=\"center\">83.3900<\/td><td class=\"has-text-align-center\" data-align=\"center\">71110<\/td><td class=\"has-text-align-center\" data-align=\"center\">3.0 mL\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">8.00<\/td><td class=\"has-text-align-center\" data-align=\"center\">NA<\/td>\n        <\/tr><tr><td>Sarstedt TC 6-well plate<\/td><td class=\"has-text-align-center\" data-align=\"center\">83.3920.005<\/td><td class=\"has-text-align-center\" data-align=\"center\">71120<\/td><td class=\"has-text-align-center\" data-align=\"center\">3.0 mL\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">8.80<\/td><td class=\"has-text-align-center\" data-align=\"center\">top left<\/td>\n        <\/tr><tr><td>Sarstedt lumox\u00ae 24-multiwell plate<\/td><td class=\"has-text-align-center\" data-align=\"center\">94.6000.014<\/td><td class=\"has-text-align-center\" data-align=\"center\">71130<\/td><td class=\"has-text-align-center\" data-align=\"center\">1.9 mL\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">1.90<\/td><td class=\"has-text-align-center\" data-align=\"center\">top left<\/td>\n        <\/tr><tr><td>Sarstedt lumox\u00ae 96-multiwell plate<\/td><td class=\"has-text-align-center\" data-align=\"center\">94.6000.024<\/td><td class=\"has-text-align-center\" data-align=\"center\">71140<\/td><td class=\"has-text-align-center\" data-align=\"center\">170 \u00b5L\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">0.34<\/td><td class=\"has-text-align-center\" data-align=\"center\">top left<\/td>\n        <\/tr><tr><td>ibidi\u00ae \u03bc-dish 35 mm, high<\/td><td class=\"has-text-align-center\" data-align=\"center\">81156<\/td><td class=\"has-text-align-center\" data-align=\"center\">71111<\/td><td class=\"has-text-align-center\" data-align=\"center\">2.5 mL\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">3.50<\/td><td class=\"has-text-align-center\" data-align=\"center\">NA<\/td>\n        <\/tr><tr><td>ibidi\u00ae \u03bc-plate 24 Well Black<\/td><td class=\"has-text-align-center\" data-align=\"center\">80241<\/td><td class=\"has-text-align-center\" data-align=\"center\">71131<\/td><td class=\"has-text-align-center\" data-align=\"center\">2.5 mL\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">1.90<\/td><td class=\"has-text-align-center\" data-align=\"center\">NA<\/td>\n        <\/tr><tr><td>Eppendorf CCCadvanced\u00ae FN1 &#8211; 6 well<\/td><td class=\"has-text-align-center\" data-align=\"center\">0038110010<\/td><td class=\"has-text-align-center\" data-align=\"center\">71150<\/td><td class=\"has-text-align-center\" data-align=\"center\">3.0 mL\/well<\/td><td class=\"has-text-align-center\" data-align=\"center\">9.40<\/td><td class=\"has-text-align-center\" data-align=\"center\">bottom right<\/td>\n        <\/tr>\n      <\/tbody>\n    <\/table>\n  <\/figure>\n  <div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-top is-style-border is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:551px;--phi-aspect-ratio:1.8584392014519;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General capture\" class=\"wp-image-31888 size-full lazyload figure__media\" width=\"1024\" height=\"551\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-1024x551.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-1024x551.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-300x161.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-768x413.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-150x81.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-512x275.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-1536x826.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-1280x689.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture.jpg 1920w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture.jpg\">\n      <\/div>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">Sterilize the <a href=\"https:\/\/phiab.com\/holomonitor\/hololids\/\">HoloLids\u2122<\/a> according to the HoloLid\u2122 sterilization and use protocol.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">Add the <strong>treatment <\/strong>to your cells. The final working volumes per well, essential for using <strong>HoloLids\u2122<\/strong>, are shown in the table above.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block\"><strong>Slide <\/strong>the cell culture <strong>vessel <\/strong>onto the Vessel <strong>holder<\/strong>, its grips facing towards you. Ensure that the vessel is parallel to the holder. There is a spring that holds the vessel in place.<\/p>\n      <p class=\"is-style-indented wp-block-paragraph block-editor-block-list__block\">\u25baWhen using multi-well plates, place them with the cut-off corner to the left.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block\"><strong>Replace <\/strong>the standard lids with the <strong>HoloLid\u2122<\/strong>.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block\"><strong>Put <\/strong>the vessel <strong>holder <\/strong>with the sample on the <strong>HoloMonitor\u00ae M4&nbsp;stage<\/strong>.<\/p>\n      <p class=\"is-style-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">Select the <strong>General Capture<\/strong> and proceed by clicking the<strong> Setup Capture <\/strong>button.<\/p>\n    <\/div>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-group alignwide is-style-curve has-phi-blue-background-color has-background is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <h2 class=\"wp-block-heading alignwide is-style-page-break has-large-font-size block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" id=\"h-experiment-setup\">\n    <strong>EXPERIMENT SETUP<\/strong>\n  <\/h2>\n<\/div>\n\n<h3 class=\"wp-block-heading alignwide is-style-numbered has-small-large-font-size\" id=\"h-basic-setup-describe-the-experiment-and-assign-treatments-to-the-wells\">Basic setup: Describe the experiment and assign treatments to the wells<\/h3>\n\n<div class=\"wp-block-group alignwide is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-style-border is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:549px;--phi-aspect-ratio:1.8652094717668;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General-capture-setup\" class=\"wp-image-31889 size-full lazyload figure__media\" width=\"1024\" height=\"549\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-1024x549.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-1024x549.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-300x161.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-768x412.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-150x80.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-512x275.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-1536x824.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-1280x687.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup.jpg 1920w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup.jpg\">\n      <\/div>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">Enter the experiment <strong>name<\/strong>, optional experiment <strong>description <\/strong>and cell types.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">Select the correct <strong>vessel map <\/strong>from the drop-down list.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block\">Map <strong>treatments and conditions<\/strong> on the vessel map. <strong>Select wells<\/strong> by marking them with the left mouse button while moving the cursor over the relevant well\/s.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block\">Add the<strong> treatment name(s)<\/strong> in the text box below the vessel map and click <strong>Add<\/strong>\/ press Enter. It is possible to add wells as <strong>individual treatments<\/strong>. Marked well\/s are light blue, selected wells will appear dark blue.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">Proceed to<strong> Capture setup<\/strong>.<\/p>\n    <\/div>\n  <\/div>\n<\/div>\n\n<h3 class=\"wp-block-heading alignwide is-style-numbered has-small-large-font-size\" id=\"h-capture-setup-select-the-experiment-time-settings-and-choose-capture-positions\">Capture setup: Select the experiment time settings and choose capture positions<\/h3>\n\n<div class=\"wp-block-group alignwide is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\"><div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-top is-style-border is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__first\" style=\"--phi-media-width:1024px;--phi-media-height:548px;--phi-aspect-ratio:1.8686131386861;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General-capture-setup-tab\" class=\"wp-image-31890 size-full lazyload figure__media\" width=\"1024\" height=\"548\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-1024x548.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-1024x548.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-300x161.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-768x411.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-150x80.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-512x274.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-1536x822.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab-1280x685.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab.jpg 1920w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-setup-tab.jpg\">\n      <\/div>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">Adjust the default settings for the<strong> duration<\/strong>, <strong>interval, <\/strong>and <strong>number of positions<\/strong>.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">Ensure that the<strong> storage requirement <\/strong>for the experiment does not exceed the computer capacity.<\/p>\n      <p class=\"is-style-indented wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">When running an experiment, <strong>data needs to be stored on the computer <\/strong>connected to the instrument. Storing data on an external drive (e.g. connected via USB or internet server) may cause data loss due to erratic USB connections or poor internet connection.<\/p>\n    <\/div>\n  <\/div>\n  <div class=\"wp-block-group alignwide is-style-float-right is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout floated-parent block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\"><div class=\"wp-block-group is-style-default is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__first floating-element\">\n      <figure class=\"wp-block-image alignleft size-large is-style-lightbox has-caption is-landscape block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:236px;--phi-aspect-ratio:4.3389830508475;\">\n        <div class=\"lightbox-image-wrapper\">\n          <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"Manual focus adjustment\" class=\"wp-image-31364 lazyload figure__media\" width=\"1024\" height=\"236\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-1024x236.png\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-1024x236.png 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-300x69.png 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-768x177.png 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-150x35.png 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-512x118.png 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment-1280x296.png 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment.png 1490w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/Manual-focus-adjustment.png\">\n        <\/div><figcaption class=\"wp-element-caption\"><strong>MANUAL FOCUS ADJUSTMENT:<\/strong> The focusing tool is located in the<strong> Controls tab<\/strong>. Move the black square or click on the Arrow buttons to move the stage up and down. Save an adjusted focus setting for the selected position by using the Apply Current Focus button. For details, consult the<a href=\"https:\/\/phiab.com\/resources\/manuals-protocols\/\"> <strong>Setup and Operational Manual<\/strong><\/a><strong>.<\/strong><\/figcaption>\n      <\/figure>\n    <\/div>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">Run a full or quick <strong>validation <\/strong>of the selected positions to ensure <strong>good image quality<\/strong>.<\/p>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block\">Click the p<strong>ositions list<\/strong> button to view the position list.<\/p>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block\"><strong>Inspect the images<\/strong> by hovering over the image icon in the list. Move the stage to that position by double-clicking the icon. If the image quality is poor, a warning sign appears. <strong>Adjust <\/strong>position location or focus if necessary.<\/p>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">When satisfied with the experiment setup, click <strong>Proceed<\/strong> <strong>to Capture<\/strong>.<\/p>\n  <\/div>\n<\/div>\n\n<h3 class=\"wp-block-heading alignwide is-style-numbered has-small-large-font-size\" id=\"h-capture-review-the-experiment-in-real-time-during-the-time-lapse\">Capture: Review the experiment in real-time during the time-lapse<\/h3>\n\n<div class=\"wp-block-group alignwide is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-center is-style-border is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:549px;--phi-aspect-ratio:1.8652094717668;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General-capture-capture-setup\" class=\"wp-image-31892 size-full lazyload figure__media\" width=\"1024\" height=\"549\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-1024x549.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-1024x549.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-300x161.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-768x412.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-150x80.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-512x275.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-1536x824.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup-1280x687.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup.jpg 1920w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-capture-setup.jpg\">\n      <\/div>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">Click <strong>Start Capture.<\/strong><\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">To stop the experiment ahead of time, click the stop button.<\/p>\n      <p class=\"is-style-indented wp-block-paragraph block-editor-block-list__block\">\u25ba Note that it is NOT possible to restart the experiment once it has been stopped.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block\">Go to the <strong>Experiments tab <\/strong>and open your ongoing experiment to preview the results during the run.<\/p>\n      <p class=\"is-style-indented wp-block-paragraph block-editor-block-list__block\">\u25baWait for the experiment to finish before starting data analysis.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">When the Experiment capture finishes, click the <strong>Show Result<\/strong> button to get directly to the <strong>Results page<\/strong>.<\/p>\n    <\/div>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-group alignwide is-style-curve has-phi-blue-background-color has-background is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <h2 class=\"wp-block-heading alignwide is-style-page-break has-large-font-size block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" id=\"h-results-analysis\">\n    <strong>RESULTS &amp; ANALYSIS<\/strong>\n  <\/h2>\n<\/div>\n\n<h3 class=\"wp-block-heading alignwide has-small-large-font-size\" id=\"h-experiments-tab\">Experiments tab<\/h3>\n\n<div class=\"wp-block-group alignwide is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-top is-style-border is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:550px;--phi-aspect-ratio:1.8618181818182;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General-capture-results-tab\" class=\"wp-image-31895 size-full lazyload figure__media\" width=\"1024\" height=\"550\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-1024x550.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-1024x550.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-300x161.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-768x413.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-150x81.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-512x275.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-1536x826.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab-1280x688.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab.jpg 1920w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-results-tab.jpg\">\n      <\/div>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">Click Experiments to see a <strong>list of the experiments<\/strong>.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\">Click on the experiment title to open an <strong>experiment summary<\/strong>.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\">Click <strong>Open <\/strong>to open the results page to view all<strong> images<\/strong>.<\/p>\n    <\/div>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-group alignwide is-style-curve has-phi-blue-background-color has-background is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <h2 class=\"wp-block-heading alignwide is-style-page-break has-large-font-size block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" id=\"h-one-experiment-multiple-results\">\n    <strong>One experiment \u2014 multiple results<\/strong>\n  <\/h2>\n<\/div>\n\n<ul class=\"is-style-checkmark wp-block-list block-editor-block-list__layout\">\n  <li class=\"block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\">This section helps to reanalyze data between different assays using HoloMonitor\u00ae App Suite software.<\/li>\n<\/ul>\n\n<h3 class=\"wp-block-heading alignwide has-small-large-font-size\" id=\"h-generating-in-depth-assay-results\">Generating In-depth Assay results<\/h3>\n\n<div class=\"wp-block-group alignwide is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout\">\n  <div class=\"wp-block-media-text alignwide has-media-on-the-right is-stacked-on-mobile is-vertically-aligned-top is-style-lightbox floated-parent block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:768px;--phi-aspect-ratio:1.3333333333333;\"><figure class=\"wp-block-media-text__media is-landscape floating-element\">\n      <a href=\"https:\/\/phiab.com\/holomonitor\/live-cell-assays\/?swcfpc=1\" class=\"figure__media figure__media-link\">\n        <div class=\"lightbox-image-wrapper\">\n          <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General-capture-reanalysis\" class=\"wp-image-31897 size-medium_large lazyload\" width=\"1024\" height=\"768\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-1024x768.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-1024x768.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-300x225.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-768x576.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-150x112.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-512x384.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis-1280x960.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis.jpg 1438w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-reanalysis.jpg\">\n        <\/div><\/a>\n    <\/figure><div class=\"wp-block-media-text__content block-editor-block-list__layout\"><p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">In the<strong> Experiment overview page<\/strong> select the In-depth application icon for the wanted result.<\/p>\n      <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\"><strong>Follow <\/strong>the respective assay protocol.<\/p>\n    <\/div>\n  <\/div>\n<\/div>\n\n<h3 class=\"wp-block-heading alignwide has-small-large-font-size\" id=\"h-generating-guided-assay-results\">Generating Guided Assay results<\/h3>\n\n<div class=\"wp-block-group alignwide is-style-float-right is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout floated-parent\"><div class=\"wp-block-group is-style-default is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__first floating-element\">\n    <figure class=\"wp-block-image alignleft size-large is-style-border is-style-lightbox has-caption is-landscape block-editor-block-list__block block-editor-block-list__first block-editor-block-list__last\" style=\"--phi-media-width:1024px;--phi-media-height:550px;--phi-aspect-ratio:1.8618181818182;\">\n      <div class=\"lightbox-image-wrapper\">\n        <img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/phiab.com\/wp-content\/themes\/phi-xxvi\/images\/placeholder.svg\" alt=\"General-capture-result\" class=\"wp-image-31898 lazyload figure__media\" width=\"1024\" height=\"550\" data-src=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-1024x550.jpg\" data-srcset=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-1024x550.jpg 1024w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-300x161.jpg 300w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-768x413.jpg 768w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-150x81.jpg 150w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-512x275.jpg 512w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-1536x826.jpg 1536w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result-1280x688.jpg 1280w, https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result.jpg 1920w\" data-sizes=\"auto\" data-full-url=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/10\/General-capture-result.jpg\">\n      <\/div><figcaption class=\"wp-element-caption\">When creating a New Guided Assay result from Cell Morphology experiment &#8211; the first analysis will take some time, as the software needs to evaluate image quality and perform image analysis.<\/figcaption>\n    <\/figure>\n  <\/div>\n  <div class=\"wp-block-group is-style-restart-numbering is-layout-flow wp-block-group-is-layout-flow block-editor-block-list__layout block-editor-block-list__block block-editor-block-list__second block-editor-block-list__last\"><p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__first\">In the <strong>Experiment overview page<\/strong> under Guided Assay Results select <strong>Create New Result<\/strong>.<\/p>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__second\"><strong>Choose the <\/strong>type of analysis in the pop-up window and name the new result.<\/p>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block\">Tick<strong> copy image analysis<\/strong> from and select the experiment to copy from. This will copy the image analysis settings from the selected result including all changes.<\/p>\n    <p class=\"is-style-indented wp-block-paragraph block-editor-block-list__block\">\u25ba For further data analysis steps, please see the respective assay protocol.<\/p>\n    <p class=\"is-style-box-numbered wp-block-paragraph block-editor-block-list__block block-editor-block-list__last\"><strong>Press create<\/strong>.<\/p>\n  <\/div>\n<\/div>\n\n<div class=\"wp-block-buttons is-layout-flex wp-block-buttons-is-layout-flex\">\n  <div class=\"wp-block-button\">\n    <a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/phiab.com\/reports\/AppSuite-4.0\/AppSuite-protocol-general-capture.pdf\">Download this protocol<\/a>\n  <\/div>\n<\/div>","protected":false},"excerpt":{"rendered":"The HoloMonitor\u00ae General Capture Assay provides time-lapse images for further analysis by any other HoloMonitor\u00ae App Suite assays.","protected":false},"author":16,"featured_media":26509,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"ub_ctt_via":"","footnotes":""},"categories":[6157],"tags":[],"featured_image_src":"https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner.jpg","author_info":{"display_name":"Haoran Yu","author_link":"https:\/\/phiab.com\/author\/hy\/"},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v21.6 (Yoast SEO v21.6) - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>HoloMonitor\u00ae General Capture Protocol - Phase Holographic Imaging<\/title>\n<meta name=\"description\" content=\"This protocol helps to set up a General Capture Assay using HoloMonitor\u00ae M4 and the HoloMonitor\u00aeApp Suite software.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/phiab.com\/protocol\/general-capture\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"General Capture Assay\" \/>\n<meta property=\"og:description\" content=\"This protocol helps to set up a General Capture Assay using HoloMonitor\u00ae M4 and the HoloMonitor\u00aeApp Suite software.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/phiab.com\/protocol\/general-capture\/\" \/>\n<meta property=\"og:site_name\" content=\"PHI\" \/>\n<meta property=\"article:published_time\" content=\"2021-10-28T12:12:10+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-11-01T10:07:15+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1536\" \/>\n\t<meta property=\"og:image:height\" content=\"512\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Haoran Yu\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Haoran Yu\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"7 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/phiab.com\/protocol\/general-capture\/\",\"url\":\"https:\/\/phiab.com\/protocol\/general-capture\/\",\"name\":\"HoloMonitor\u00ae General Capture Protocol - Phase Holographic Imaging\",\"isPartOf\":{\"@id\":\"https:\/\/phiab.com\/#website\"},\"datePublished\":\"2021-10-28T12:12:10+00:00\",\"dateModified\":\"2023-11-01T10:07:15+00:00\",\"author\":{\"@id\":\"https:\/\/phiab.com\/#\/schema\/person\/0c2a50176057b8e30980ec6fbbd36566\"},\"description\":\"This protocol helps to set up a General Capture Assay using HoloMonitor\u00ae M4 and the HoloMonitor\u00aeApp Suite software.\",\"breadcrumb\":{\"@id\":\"https:\/\/phiab.com\/protocol\/general-capture\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\/\/phiab.com\/protocol\/general-capture\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\/\/phiab.com\/protocol\/general-capture\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\/\/phiab.com\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"General Capture Assay\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\/\/phiab.com\/#website\",\"url\":\"https:\/\/phiab.com\/\",\"name\":\"PHI\",\"description\":\"Live Cell Imaging Made Easy\",\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\/\/phiab.com\/#\/schema\/person\/0c2a50176057b8e30980ec6fbbd36566\",\"name\":\"Haoran Yu\",\"url\":\"https:\/\/phiab.com\/author\/hy\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"HoloMonitor\u00ae General Capture Protocol - Phase Holographic Imaging","description":"This protocol helps to set up a General Capture Assay using HoloMonitor\u00ae M4 and the HoloMonitor\u00aeApp Suite software.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/phiab.com\/protocol\/general-capture\/","og_locale":"en_US","og_type":"article","og_title":"General Capture Assay","og_description":"This protocol helps to set up a General Capture Assay using HoloMonitor\u00ae M4 and the HoloMonitor\u00aeApp Suite software.","og_url":"https:\/\/phiab.com\/protocol\/general-capture\/","og_site_name":"PHI","article_published_time":"2021-10-28T12:12:10+00:00","article_modified_time":"2023-11-01T10:07:15+00:00","og_image":[{"width":1536,"height":512,"url":"https:\/\/phiab.com\/wp-content\/uploads\/2021\/05\/blue-cells_white-bg_banner.jpg","type":"image\/jpeg"}],"author":"Haoran Yu","twitter_card":"summary_large_image","twitter_misc":{"Written by":"Haoran Yu","Est. reading time":"7 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/phiab.com\/protocol\/general-capture\/","url":"https:\/\/phiab.com\/protocol\/general-capture\/","name":"HoloMonitor\u00ae General Capture Protocol - Phase Holographic Imaging","isPartOf":{"@id":"https:\/\/phiab.com\/#website"},"datePublished":"2021-10-28T12:12:10+00:00","dateModified":"2023-11-01T10:07:15+00:00","author":{"@id":"https:\/\/phiab.com\/#\/schema\/person\/0c2a50176057b8e30980ec6fbbd36566"},"description":"This protocol helps to set up a General Capture Assay using HoloMonitor\u00ae M4 and the HoloMonitor\u00aeApp Suite software.","breadcrumb":{"@id":"https:\/\/phiab.com\/protocol\/general-capture\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/phiab.com\/protocol\/general-capture\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/phiab.com\/protocol\/general-capture\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/phiab.com\/"},{"@type":"ListItem","position":2,"name":"General Capture Assay"}]},{"@type":"WebSite","@id":"https:\/\/phiab.com\/#website","url":"https:\/\/phiab.com\/","name":"PHI","description":"Live Cell Imaging Made Easy","inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/phiab.com\/#\/schema\/person\/0c2a50176057b8e30980ec6fbbd36566","name":"Haoran Yu","url":"https:\/\/phiab.com\/author\/hy\/"}]}},"_links":{"self":[{"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/posts\/31876"}],"collection":[{"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/users\/16"}],"replies":[{"embeddable":true,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/comments?post=31876"}],"version-history":[{"count":3,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/posts\/31876\/revisions"}],"predecessor-version":[{"id":39186,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/posts\/31876\/revisions\/39186"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/media\/26509"}],"wp:attachment":[{"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/media?parent=31876"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/categories?post=31876"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/phiab.com\/wp-json\/wp\/v2\/tags?post=31876"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}