OLIGO

OLIGO Primer Analysis Software is the essential tool for designing and analyzing sequencing and PCR primers, synthetic genes.

Besides that it's also used for various kinds of probes including siRNA and molecular beacons.
It is also an invaluable tool for site directed mutagenesis.Based on the most up-to date nearest neighbor thermodynamic data, Oligo's search algorithms find optimal primers for PCR, including TaqMan, highly multiplexed, consensus or degenerate primers. Multiple file batch processing is possible.