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Interrelationships between increased vascular permeability and acute neuronal damage following temperature-controlled brain ischemia in rats

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Summary

This study examined regional patterns of increased vascular permeability and morphological indicators of acute neuronal injury following normothermic and mildly hyperthermic forebrain ischemia. Rats underwent 20 min of four-vessel occlusion during which intraischemic brain temperature was maintained at either 37°C or 39°C. At 45-min recirculation, the blood-brain barrier (BBB)-tracer horseradish peroxidase was injected and rats were perfusion-fixed at 1-h recirculation for light and electron microscopic analysis. In normothermic and hyperthermic rats, sites of increased vascular permeability were spatially correlated with dark shrunken type IV neurons. Neuronal alterations within cortical, hippocampal, striatal, and thalamic areas ranged from mild cytoplasmic vacuolation and mitochondrial swelling to severe cytoplasmic shrinkage and increased density. Although dark shrunken neurons were routinely associated with permeable blood vessels in both temperature groups, dark neurons were not detected in regions demonstrating an intact BBB. Following normothermic brain ischemia, the appearance of dark shrunken neurons was restricted to the cerebral cortex and striatum. In both temperature groups, luminal leukocytes were detected within otherwise well-perfused forebrain microvascular beds. Our studies suggest a close interrelationship between postischemic microvascular abnormalities, including increased vascular permeability, and morphological indicators of acute neuronal injury following brain ischemia.

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Authors and Affiliations

  1. Cerebral Vascular Disease Research Center, Department of Neurology (D4-5), University of Miami School of Medicine, P.O. Box 016960, 33101, Miami, FL, USA

    W. D. Dietrich, M. Halley, I. Valdes & R. Busto

  2. Cerebral Vascular Disease Research Center, Department of Anatomy and Cell Biology, University of Miami School of Medicine, P.O. Box 016960, 33101, Miami, FL, USA

    W. D. Dietrich

Authors
  1. W. D. Dietrich
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  2. M. Halley
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  3. I. Valdes
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  4. R. Busto
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Additional information

Supported by USPHS Grants NS-05820 and NS-27127 and by the American Heart Association Grant-in-Aid 90–1133, with funds contributed by the Florida Affiliate.

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Dietrich, W.D., Halley, M., Valdes, I. et al. Interrelationships between increased vascular permeability and acute neuronal damage following temperature-controlled brain ischemia in rats. Acta Neuropathol 81, 615–625 (1991). https://doi.org/10.1007/BF00296371

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  • DOI: https://doi.org/10.1007/BF00296371

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