The test case for this is pretty simple, access the last element of a tuple using -1.
$ cat t.py
(1,)[-1]
$ python2.7 t.py
$ python3.5 t.py
$ mypy --py2 --fast-parser t.py
t.py:1: error: Tuple index out of range
$ mypy --fast-parser t.py
$ mypy --py2 t.py
$