I think I narrowed down the issue a bit.
All entry points are broken (not just pytest) on macOS.
This boils down to the fact that the shebang in the entry points scripts refer to .pyodide-xbuildenv-0.26.0/0.26.0/xbuildenv/pyodide-root/dist/python which itself is a script.
macOS only accepts binaries for the shebang. So the shebang is simply ignored with bash/zsh simply trying to execute remaining lines which gives the error message:

/private/var/folders/hb/2n_7f3yn20v06lzz3129dtw80000gn/T/cibw-run-zmblysfg/cp312-pyodide_wasm32/venv-test/bin/pytest: line 3: import: command not found
/private/var/folders/hb/2n_7f3yn20v06lzz3129dtw80000gn/T/cibw-run-zmblysfg/cp312-pyodide_wasm32/venv-test/bin/pytest: line 4: import: command not found
/private/var/folders/hb/2n_7f3yn20v06lzz3129dtw80000gn/T/cibw-run-zmblysfg/cp312-pyodide_wasm32/venv-test/bin/pytest: line 5: from: command not found
/private/var/folders/hb/2n_7f3yn20v06lzz3129dtw80000gn/T/cibw-run-zmblysfg/cp312-pyodide_wasm32/venv-test/bin/pytest: line 7: syntax error near unexpected token `('
/private/var/folders/hb/2n_7f3yn20v06lzz3129dtw80000gn/T/cibw-run-zmblysfg/cp312-pyodide_wasm32/venv-test/bin/pytest: line 7: `    sys.argv[0] = re.sub(r'(-script\.pyw|\.exe)?$', '', sys.argv[0])'

Uh oh!

Mac builds: Pyodide Venv: pytest breaks when python -m pytest does not #4802

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Mac builds: Pyodide Venv: `pytest` breaks when `python -m pytest` does not #4802