These are the H1-7 CRISPR Screening Libraries.
They were processed using fxtools
to make compatible for sgRNA counts mapping with sgcount.
Each library was mapped the following way - using H1 as an example:
# convert to upper case
fxtools upper -i h1_lib.fa -o h1_lib.upper.fa
# remove duplicate sgRNAs
fxtools unique -i h1_lib.upper.fa -o h1_lib.unique.fa
# extract variable region from library (remove adapters)
fxtools extract-variable -i h1_lib.unique.fa -o h1_lib.var.fa -z 0.5
# create a gene -> sgRNA mapping
fxtools sgrna-table -i h1_lib.var.fa -o h1_lib.g2s.txt