Dear Canu team,
We are working with small DNA fragments (400bp > ,< 5kb)coming from amplicons and we do not always have a reference to align them and call a consensus. In certain cases we need to assemble them de novo. We have tried to assemble these small fragments with Canu but it seems that we cannot find the right parameters to obtain a correct contig. Do you know if your software can assemble small DNA fragments? If so could you recommend parameters that we should tweak to make it work?
best regards,
Loïc
Dear Canu team,
We are working with small DNA fragments (400bp > ,< 5kb)coming from amplicons and we do not always have a reference to align them and call a consensus. In certain cases we need to assemble them de novo. We have tried to assemble these small fragments with Canu but it seems that we cannot find the right parameters to obtain a correct contig. Do you know if your software can assemble small DNA fragments? If so could you recommend parameters that we should tweak to make it work?
best regards,
Loïc