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tmma - MA plots and TMM for python

Build Status

Based on the implementation in EdgeR Bioconductor package.

Installation

tmma can be installed through pip:

pip install git+https://github.com/lukauskas/tmma.git

Development installation

For development, please clone the repository:

git clone https://github.com/lukauskas/tmma.git

and then install package with (-e) flag set and optional test dependencies.

pip install -e .[test]

Running tests

If you have installed the development version of the package, you can run the tests with the following command:

python -m unittest discover -s tests/

Usage

See example based on the Arabidopsis dataset from edgeR user guide, available in the examples directory

References

  • Robinson, M., McCarthy, D., Smyth, G. (2009). edgeR: a Bioconductor package for differential expression analysis of digital gene expression data Bioinformatics 26(1), 139-140. https://dx.doi.org/10.1093/bioinformatics/btp616
  • Robinson, M., Oshlack, A. (2010). A scaling normalization method for differential expression analysis of RNA-seq data Genome Biology 11(3), R25. https://dx.doi.org/10.1186/gb-2010-11-3-r25
  • McCarthy, D., Chen, Y., Smyth, G. (2012). Differential expression analysis of multifactor RNA-Seq experiments with respect to biological variation Nucleic Acids Research 40(10), 4288-4297. https://dx.doi.org/10.1093/nar/gks042

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MA plots and TMM for python

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