Some incomplete coordinates for sentence elements #811
Description
For this example (preprint):
Uploading document_sentence_segmentation_issues.pdf…
we have some incomplete bounding boxes for coordinates at sentence-level, see the 5 last sentences of this paragraph:
<div
xmlns="http://www.tei-c.org/ns/1.0">
<head coords="23,54.00,212.69,163.38,11.14">ODD-luciferase activity assaay</head>
<p>
<s coords="23,54.00,240.29,504.00,11.14;23,54.00,267.89,158.47,11.14">The ODD-luciferase construct with pcDNA3.1 plasmid vector was constructed as previously described
<ref type="bibr" coords="23,108.63,267.89,92.50,11.14" target="#b42">(Safran et al. 2006</ref>).
</s>
<s coords="23,215.06,267.89,342.94,11.14;23,54.00,295.49,504.01,11.14;23,54.00,323.09,222.14,11.14">The proline p402 and p564 present within the oxygen degradation domain (ODD) of HIF1α, when hydroxylated by HIF-PHDs, allow its binding to the VHL protein that target it for proteasomal degradation.</s>
<s coords="23,279.87,323.09,278.13,11.14;23,54.00,350.69,354.71,11.14">In this way, the stabilization of ODD can be used as a marker of HIF1α stability
<ref type="bibr" coords="23,197.48,350.69,95.96,11.14" target="#b42">(Safran et al. 2006</ref>
<ref type="bibr" coords="23,293.44,350.69,115.26,11.14" target="#b48">, Smirnova et al. 2010</ref>.
</s>
<s coords="23,408.71,350.69,11.34,11.14">Because of the luciferase tagged with ODD, the increase in ODD stability leads to a proportional increase in the luciferase activity and this provides a very good way of measuring the HIF1α stability in a quantitative manner with a wide dynamic range.</s>
<s coords="23,423.35,350.69,46.70,11.14">To this end, we used SH-SY5Y cells stably expressing ODDluciferase.</s>
<s coords="23,473.35,350.69,10.01,11.14">These cells were made by co-transfecting ODD-luciferase plasmid along with a puromycin resistance plasmid in SH-SY5Y cells and stably transfected cells were positively selected in presence of 4μg/ml of puromycin.</s>
<s coords="23,486.66,350.69,71.34,11.14">Luciferase activity was measured by luciferase assay kit (promega) using an LMaxII TM microplate luminometer (molecular Devices).</s>
<s coords="23,54.00,378.29,36.70,11.14">ODDluciferase activity was normalized to the protein content of each well measured by Bio-Rad DC TM protein assay kit.</s>
</p>
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