Search results

Metabarcoding data analysis of the V3–V4 region using the MiniSeq platform.

Status: Open.

#2183 In benjjneb/dada2;

· Paul-Fernandez-C opened on Mar 11, 2026

PacBio full-length 16S CCS data: almost all reads unique during dereplication and very few reads retained after denoising

Status: Open.

#2182 In benjjneb/dada2;

· alitcd opened on Mar 6, 2026

Low number of ASVs assigned to phylum

Status: Open.

#2181 In benjjneb/dada2;

· murheisa opened on Mar 3, 2026

How to choose truncation length from plotQualityProfile() for 16S V3–V4 (341F/805R) MiSeq i100 data?

Status: Open.

#2180 In benjjneb/dada2;

· biohp90 opened on Feb 24, 2026

Visualization of read quality profiles plot - thin heatmap

Status: Open.

#2178 In benjjneb/dada2;

· mmladinic opened on Feb 11, 2026

Mismatched R1/R2 PAIRS FOUND (these will crash filterAndTrim)

Status: Open.

#2177 In benjjneb/dada2;

· Kumaratenna opened on Feb 5, 2026

Issue with DADA2: Low ASV numbers in PacBio full-length ITS data

Status: Open.

#2175 In benjjneb/dada2;

· cherry52HZ opened on Feb 5, 2026

Integrating V3-V4 and V4 16S datasets for large-scale meta-analysis: Is Greengenes2 the optimal choice for cross-region alignment?

Status: Open.

#2173 In benjjneb/dada2;

· wanghang7410 opened on Jan 29, 2026

cannot remove primers on reverse reads using cutadapt

Status: Open.

#2172 In benjjneb/dada2;

· kdbchau opened on Jan 8, 2026

error in dada2 - error rates could not be estimated

Status: Open.

#2171 In benjjneb/dada2;

· lejlapasic-ctrl opened on Dec 29, 2025

Discrepancy between number of ASVs and taxonomic assignments

Status: Open.

#2170 In benjjneb/dada2;

· judithsantano opened on Dec 16, 2025

Simple mapping of original read IDs (Headers) to final DADA2 ASVs for validation purposes

Status: Open.

#2168 In benjjneb/dada2;

· CassandraHjo opened on Dec 15, 2025