Hi I noticed that for some DMS studies there are EVmutation scores for mutations that do not appear to be in focus columns from the MSAs that you provided. Is EVmutation using a different MSA?

As an example for the BLAT_ECOLX_Stiffler_2015 dataset, EVMutation has unique scores for mutations at position 24:

  mutant  EVmutation
0   H24C   -7.206646
1   H24Y   -5.784716
2   H24W   -5.258699
3   H24V   -5.273463
4   H24T   -3.646145

However, in the MSA file the WT sequence is msiqhfrvalipffaafclpvfahpetlvkvkdaedqlgARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLS RVdagQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGgPKELTAFLHNMGDHVTRL DRWEPelneaiPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGS RGIIAALGPDGKPSrIVVIYTTGSQatmdernrqiaeigaslikhw

Position 24 is a non-match column and is filtered out during MSA processing. In this case how are scores computed for these mutations?

Thanks!