You need to have python 3.6+ installed. The required python dependencies can be installed by

pip3 install --user -r requirements.txt

We also require ViennaRNA suite to be installed on your machine. The instructions to do so can be found here.

TIsigner can also check for terminators if you have Infernal installed on your machine. Instructions to install Infernal can be found here.

Once you've installed the dependencies, you can enter the following command:

python3 tisigner.py

The local web server will run on http://0.0.0.0:5000/.

TIsigner runs at http://0.0.0.0:5000/tisigner.

SoDoPE runs at http://0.0.0.0:5000/sodope.

If you see something unexpected, please report us or open an issue.