DRSC/TRiP Functional Genomics Resources & DRSC-BTRR

DRSC

The DRSC supports Drosophila cell screens using RNAi or CRISPR, generates new cell lines, and applies CRISPR screen tech to mosquito and other arthropod cell lines.

GFP-tagged and newly isolated Drosophila cell lines generated in the project funded by R24 OD019847 (to Perrimon, Mohr, and Simcox), showing GFP localized to specific organelles and example tissue-type-specific cell lines such as a muscle-like cell line

TRiP

The TRiP generates RNAi and CRISPR fly stocks for gene knockdown, knockout, and over-expression, and innovates in related areas of new in vivo resource development.

Top of a vial of Drosophila showning adult flies; the TRiP has generated many transgenic Drosophila fly stocks for knockdown, knockout, binary expression, and other uses

Online Tools

Our bioinformatics suite includes tools for identifying genes, orthologs, and reagents; analyzing, visualizing, and mining datasets; and more.

Screenshot of a grid layout of icons and links to our suite of online tools, used to demonstrate that we have a large suite of available online tools

Please Note: This website is now considered a legacy website and is not regularly updated. 

We invite you to view our new Arthropod Cell Screening Facility (ACSF) website for information about current initiatives and goals, current publication lists, and more. We will retain this site as long as feasible so that the community continues to have access to DRSC protocols, TRiP info, and more.

The Drosophila RNAi Screening Center (DRSC) & Transgenic RNAi Project (TRiP)-Functional Genomics Resources (DRSC/TRiP-FGR) at Harvard Medical School, also known as the Drosophila Research & Screening Center-Biomedical Technology Research Resource (DRSC-BTRR), has been developing technology and resources for the Drosophila and broader research community since 2004.

Recent News

DIOPT online ortholog search tool updated
We recently updated our DIOPT online ortholog search tool. Notable changes include addition of predictions for the black-legged tick, Ixodes scapularis, and removal of an outdated ortholog prediction set. Please see the attached PDF for a visual summary...

PANGEA online tool updated
We recently updated our PANGEA gene set enrichment analysis tool. The tool now supports gene set enrichment analysis for the malaria mosquito Anopheles gambiae and the black-legged tick Ixodes scapularis. In addition, we have added new gene sets for gene...
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Recent Preprints & Publications

2025

Baolong Xia, Ah-Ram Kim, Feimei Liu, Myeonghoon Han, Emily Stoneburner, Stephanie Makdissi, Francesca Di Cara, Stephanie E Mohr, Aaron Ring, and Norbert Perrimon. 2025. “Phage-Displayed Synthetic Library and Screening Platform for Nanobody Discovery.”. ELife, 14. doi:10.7554/eLife.105887.
Baolong Xia, Ah-Ram Kim, Feimei Liu, Myeonghoon Han, Emily Stoneburner, Stephanie Makdissi, Francesca Di Cara, Stephanie E Mohr, Aaron Ring, and Norbert Perrimon. 2025. “Phage-Displayed Synthetic Library and Screening Platform for Nanobody Discovery.”. ELife, 14. doi:10.7554/eLife.105887.
Justin A Bosch, Pierre Michel Jean Beltran, Cooper Cavers, James Thai LaGraff, Randy Melanson, Ankita Singh, Weihang Chen, Yanhui Hu, Sudhir Gopal Tattikota, Ying Liu, Yousuf Hashmi, John M Asara, Tess Branon, Alice Y Ting, Steven A Carr, and Norbert Perrimon. 2025. “Multi-Omic Mapping of Protein Secretomes Reveals Tissue-Specific Origins and Inter-Organ Trafficking.”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2025.07.09.659702.
Justin A Bosch, Pierre Michel Jean Beltran, Cooper Cavers, James Thai LaGraff, Randy Melanson, Ankita Singh, Weihang Chen, Yanhui Hu, Sudhir Gopal Tattikota, Ying Liu, Yousuf Hashmi, John M Asara, Tess Branon, Alice Y Ting, Steven A Carr, and Norbert Perrimon. 2025. “Multi-Omic Mapping of Protein Secretomes Reveals Tissue-Specific Origins and Inter-Organ Trafficking.”. BioRxiv : The Preprint Server for Biology. doi:10.1101/2025.07.09.659702.
Raghuvir Viswanatha, Samuel Entwisle, Yanhui Hu, Ah-Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie E Mohr, and Norbert Perrimon. 2025. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. Nature Communications, 16, 1, Pp. 6498. doi:10.1038/s41467-025-61692-3.
Raghuvir Viswanatha, Samuel Entwisle, Yanhui Hu, Ah-Ram Kim, Kelly Reap, Matthew Butnaru, Mujeeb Qadiri, Stephanie E Mohr, and Norbert Perrimon. 2025. “Higher Resolution Pooled Genome-Wide CRISPR Knockout Screening in Drosophila Cells Using Integration and Anti-CRISPR (IntAC).”. Nature Communications, 16, 1, Pp. 6498. doi:10.1038/s41467-025-61692-3.
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