{"id":629,"date":"2018-06-08T18:34:37","date_gmt":"2018-06-08T18:34:37","guid":{"rendered":"https:\/\/docs.flowjo.com\/seqgeq\/?page_id=629"},"modified":"2020-03-05T23:59:13","modified_gmt":"2020-03-05T23:59:13","slug":"analysis-workflows","status":"publish","type":"page","link":"https:\/\/docs.flowjo.com\/seqgeq\/analysis-workflows\/","title":{"rendered":"Analysis Workflows"},"content":{"rendered":"<p class=\"lead\"><span style=\"font-weight: 400\">There are at least as many different possibilities when planning an analysis strategy in SeqGeq, as there are cell types to explore. <\/span><\/p>\n<p><span style=\"font-weight: 400\">In the following pages we detail some broad possible directions a researcher might take, and considerations involved for each.<\/span><\/p>\n<p>&nbsp;<\/p>\n<h1><span style=\"font-weight: 400\">Overview<\/span><\/h1>\n<p><span style=\"font-weight: 400\">The tack used for a given analysis should be tailored to the model investigated, the questions a researcher is attempting to answer, and the technology used to acquire the data. <\/span><\/p>\n<p>Keep in mind that each step in an analysis protocol can be re-iterated over deeper subpopulations discovered, as well as the initial sample. You won\u2019t always get the best results on your first try &#8211; so try adjusting the parameter quality gating for downstream analysis, or adjusting settings for dimensionality reduction visualizations to improve on cluster separation.<\/p>\n<p>Don\u2019t be afraid to mix and match techniques! These workflows are only suggestions and a robust analysis protocol may use tool described in any of the following workflows.<\/p>\n<p>&nbsp;<\/p>\n<h1><span style=\"font-weight: 400\">Workflows<\/span><\/h1>\n<ul>\n<li><a href=\"https:\/\/docs.flowjo.com\/seqgeq\/analysis-workflows\/approach\/\"><span style=\"font-weight: 400\">Initial Approach<\/span><\/a><\/li>\n<li><span style=\"font-weight: 400\"><a href=\"https:\/\/docs.flowjo.com\/seqgeq\/analysis-workflows\/bulk-sequencing\/\">Bulk Sequencing<\/a><\/span><\/li>\n<li><a href=\"https:\/\/docs.flowjo.com\/seqgeq\/analysis-workflows\/combining-flow-and-single-cell-sequencing\/\"><span style=\"font-weight: 400\">Mixed Flow and Single-Cell Sequencing Analyses<\/span><\/a><\/li>\n<\/ul>\n<p><strong>Coming Soon!<\/strong><\/p>\n<ul>\n<li><span style=\"font-weight: 400\">Barcoded Antibody Sequencing<\/span><\/li>\n<li><span style=\"font-weight: 400\">High Parameter Flow<\/span><\/li>\n<\/ul>\n<p><span style=\"font-weight: 400\">If you\u2019re still having trouble getting results from your data, or choosing a method of approach, don\u2019t hesitate to reach out: <a href=\"mailto:seqgeq@flowjo.com\">seqgeq@flowjo.com<\/a><\/span><\/p>\n","protected":false},"excerpt":{"rendered":"<p>There are at least as many different possibilities when planning an analysis strategy in SeqGeq, as there are cell types to explore. In the following pages we detail some broad possible directions a researcher might take, and considerations involved for each. &nbsp; Overview The tack used for a given analysis should be tailored to the&#8230;  <a href=\"https:\/\/docs.flowjo.com\/seqgeq\/analysis-workflows\/\" class=\"more-link\" title=\"Read Analysis Workflows\">Read more &raquo;<\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"parent":266,"menu_order":53,"comment_status":"closed","ping_status":"closed","template":"home-seqgeq.php","meta":{"footnotes":""},"tags":[40],"class_list":["post-629","page","type-page","status-publish","hentry","tag-seqgeq"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Analysis Workflows - FlowJo Documentation<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/docs.flowjo.com\/seqgeq\/analysis-workflows\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Analysis Workflows - FlowJo Documentation\" \/>\n<meta property=\"og:description\" content=\"There are at least as many different possibilities when planning an analysis strategy in SeqGeq, as there are cell types to explore. 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