I'm trying to keep it to civilians (ie non-drag queens). A few have slipped in (ie Willam Belli, Michael Andrews in Angel 2, Derek McKinnon, a professional Nova Scotian-born Montreal queen deliberately cast in Terror Train for his femulation technique), but it tends to be a blurring of the lines (esp. pre-2000 - a lot of people who identified as drag queens/transvestites would identify as women now). I only really put people I knew who are not trans-identifying.

I included Belli maybe because he/she is very clear they're not trans, despite playing Candy.

And some of these may be not quite trans characters (I put Terror Train, cos Kenny is living as a woman).
Christopher Morley is absent, because AFAIK they did identify as she/he/it, and was gender-nonconforming till their death in early 2024, age 72.

I am including non-drag actors as drag queens to some extent, simply cos a lot of filmmakers didn't know the difference.

A few trans-masc cases slipped in because they have the actor present as female (My Dearest Senorita has Jose Luis Lopez Vasquez as an intersex woman who transitions to male, Private Parts has a male actor play a girl forcibly transitioned to male and no one's quite sure if Z-Man in Beyond the Valley of the Dolls is transmasc or transfemme, because it seems different people had different ideas).