{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T13:33:18Z","timestamp":1768397598415,"version":"3.49.0"},"reference-count":20,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T00:00:00Z","timestamp":1256860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>A one-step, single use, disposable Alkaline Phosphatase (ALP) biosensor has been developed. It is based on the detection of phenol produced by an ALP enzymatic reaction. It can operate at 25 \u00b0C in a pH 10 medium. It measures ALP of 0\u2013300 IU\/L. The permissible concentrations of glucose, ascorbic acid and urea without interference are 10 mM\/L, 5 mg\/L and 400 mg\/L, respectively. Experimental results are compared to those obtained by spectrophotometric measurements in bovine serum. Excellent linearity between the biosensor outputs and the ALP concentrations exists. The agreement between the measurements of this biosensor and the spectrophotometer is also outstanding.<\/jats:p>","DOI":"10.3390\/s91108709","type":"journal-article","created":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T11:49:08Z","timestamp":1256903348000},"page":"8709-8721","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":53,"title":["The Detection of Alkaline Phosphatase Using an Electrochemical Biosensor in a Single-Step Approach"],"prefix":"10.3390","volume":"9","author":[{"given":"Joanne H.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Biology, Brown University, 171 Meeting Street, Providence, RI 02912, USA"}]},{"given":"Kevin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Medicine, University of Cincinnati, 231 Albert B. Sabin Way, Cincinnati, OH 45267, USA"}]},{"given":"Brandon","family":"Bartling","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA"}]},{"given":"Chung-Chiun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, OH 44106, USA"}]}],"member":"1968","published-online":{"date-parts":[[2009,10,30]]},"reference":[{"key":"ref_1","unstructured":"Fishman, W.H., and Stigbrand, T. (1983, January September). Human alkaline phosphatases. Umea, Sweden."},{"key":"ref_2","unstructured":"Favus, M.J. (2006). Primer on the metabolic bone diseases and disorders of mineral metabolism, American Society for Bone and Mineral Research."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"McComb, R.B., Bowers, G.N., and Posen, S. (1979). 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