{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T23:03:47Z","timestamp":1771023827913,"version":"3.50.1"},"reference-count":43,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2017,2,9]],"date-time":"2017-02-09T00:00:00Z","timestamp":1486598400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016M3C7A1905477"],"award-info":[{"award-number":["2016M3C7A1905477"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sensors"],"abstract":"<jats:p>In this paper, we introduce a dry non-invasive multi-channel sensor for measuring brainwaves on the scalps of mice. The research on laboratory animals provide insights to various practical applications involving human beings and other animals such as working animals, pets, and livestock. An experimental framework targeting the laboratory animals has the potential to lead to successful translational research when it closely resembles the environment of real applications. To serve scalp electroencephalography (EEG) research environments for the laboratory mice, the dry non-invasive scalp EEG sensor with sixteen electrodes is proposed to measure brainwaves over the entire brain area without any surgical procedures. We validated the proposed sensor system with visual evoked potential (VEP) experiments elicited by flash stimulations. The VEP responses obtained from experiments are compared with the existing literature, and analyzed in temporal and spatial perspectives. We further interpret the experimental results using time-frequency distribution (TFD) and distance measurements. The developed sensor guarantees stable operations for in vivo experiments in a non-invasive manner without surgical procedures, therefore exhibiting a high potential to strengthen longitudinal experimental studies and reliable translational research exploiting non-invasive paradigms.<\/jats:p>","DOI":"10.3390\/s17020326","type":"journal-article","created":{"date-parts":[[2017,2,10]],"date-time":"2017-02-10T05:27:09Z","timestamp":1486704429000},"page":"326","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Development and Experimental Validation of a Dry Non-Invasive Multi-Channel Mouse Scalp EEG Sensor through Visual Evoked Potential Recordings"],"prefix":"10.3390","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1982-8591","authenticated-orcid":false,"given":"Donghyeon","family":"Kim","sequence":"first","affiliation":[{"name":"School of Electrical Engineering and Computer Science, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea"}]},{"given":"Chanmi","family":"Yeon","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea"}]},{"given":"Kiseon","family":"Kim","sequence":"additional","affiliation":[{"name":"School of Electrical Engineering and Computer Science, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea"}]}],"member":"1968","published-online":{"date-parts":[[2017,2,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1016\/j.neuron.2014.06.003","article-title":"Basic neuroscience research with nonhuman primates: A small but indispensable component of biomedical research","volume":"82","author":"Roelfsema","year":"2014","journal-title":"Neuron"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jneumeth.2008.03.001","article-title":"A glue-based, screw-free method for implantation of intra-cranial electrodes in young mice","volume":"171","author":"Wu","year":"2008","journal-title":"J. Neurosci. Methods"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1016\/j.neuroimage.2008.05.016","article-title":"A mouse model for studying large-scale neuronal networks using EEG mapping techniques","volume":"42","author":"Quairiaux","year":"2008","journal-title":"NeuroImage"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/0165-3806(85)90066-5","article-title":"Early postnatal development of EEG and sleep-waking cycle in two inbred mouse strains","volume":"354","author":"Daszuta","year":"1985","journal-title":"Brain Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"9574","DOI":"10.1523\/JNEUROSCI.5995-10.2011","article-title":"Functional development of large-scale sensorimotor cortical networks in the brain","volume":"31","author":"Quairiaux","year":"2011","journal-title":"J. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.neuroscience.2004.11.018","article-title":"Sleep and circadian abnormalities in a transgenic mouse model of Alzheimer\u2019s disease: A role for cholinergic transmission","volume":"131","author":"Wisor","year":"2005","journal-title":"Neuroscience"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1483","DOI":"10.1038\/srep01483","article-title":"Automated identification of multiple seizure-related and interictal epileptiform event types in the EEG of mice","volume":"3","author":"Bergstrom","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1038\/nn1754","article-title":"Reduced sodium current in GABAergic interneurons in a mouse model of severe myoclonic epilepsy in infancy","volume":"9","author":"Frank","year":"2006","journal-title":"Nat. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7695","DOI":"10.1523\/JNEUROSCI.22-17-07695.2002","article-title":"Anatomical, physiological, and pharmacological characteristics of histidine decarboxylase knock-out mice: Evidence for the role of brain histamine in behavioral and sleep-wake control","volume":"22","author":"Parmentier","year":"2002","journal-title":"J. Neurosci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1111\/j.1528-1167.2005.00287.x","article-title":"Pharmacologic Evidence for Abnormal Thalamocortical Functioning in GABAA Receptor \u03b23 Subunit-Deficient Mice, a Model of Angelman Syndrome","volume":"46","author":"Handforth","year":"2005","journal-title":"Epilepsia"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nrn3241","article-title":"The origin of extracellular fields and currents\u2014EEG, ECoG, LFP and spikes","volume":"13","author":"Anastassiou","year":"2012","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"736","DOI":"10.3758\/BRM.41.3.736","article-title":"An improved screw-free method for electrode implantation and intracranial electroencephalographic recordings in mice","volume":"41","author":"Wu","year":"2009","journal-title":"Behav. Res. Methods"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/S0165-0270(03)00172-9","article-title":"Design of a twin tetrode microdrive and headstage for hippocampal single unit recordings in behaving mice","volume":"129","author":"Jeantet","year":"2003","journal-title":"J. Neurosci. Methods"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0306-4522(02)00669-3","article-title":"Hippocampal network patterns of activity in the mouse","volume":"116","author":"Buhl","year":"2003","journal-title":"Neuroscience"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.jneumeth.2006.05.031","article-title":"Miniature microdrive\u2013headstage assembly for extracellular recording of neuronal activity with high-impedance electrodes in freely moving mice","volume":"158","author":"Korshunov","year":"2006","journal-title":"J. Neurosci. Methods"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.jneumeth.2005.10.016","article-title":"A MEMS fabricated flexible electrode array for recording surface field potentials","volume":"153","author":"Hollenberg","year":"2006","journal-title":"J. Neurosci. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"14964","DOI":"10.1038\/srep14964","article-title":"Characterization of spatio-temporal epidural event-related potentials for mouse models of psychiatric disorders","volume":"5","author":"Wang","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3425","DOI":"10.1109\/TBME.2013.2263226","article-title":"An electroplating-free and minimal noise polyimide microelectrode for recording auditory evoked potentials from the epicranius","volume":"60","author":"Kim","year":"2013","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_19","first-page":"e2562","article-title":"High-density EEG recordings of the freely moving mice using polyimide-based microelectrode","volume":"47","author":"Lee","year":"2011","journal-title":"J. Vis. Exp."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1825","DOI":"10.1152\/jn.00188.2010","article-title":"High resolution electroencephalography in freely moving mice","volume":"104","author":"Choi","year":"2010","journal-title":"J. Neurophysiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1007\/s00542-010-1152-3","article-title":"A dry release of polyimide electrodes using Kapton film and application to EEG signal measurements","volume":"17","author":"Baek","year":"2011","journal-title":"Microsyst. Technol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/S0165-0270(00)00164-3","article-title":"Epicranial sensory evoked potential recordings for repeated assessment of cortical functions in mice","volume":"97","author":"Troncoso","year":"2000","journal-title":"J. Neurosci. Methods"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1093\/ilar\/ilu047","article-title":"The significance of meaning: why do over 90% of behavioral neuroscience results fail to translate to humans, and what can we do to fix it?","volume":"55","author":"Garner","year":"2014","journal-title":"ILAR J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"12847","DOI":"10.3390\/s140712847","article-title":"Dry EEG electrodes","volume":"14","year":"2014","journal-title":"Sensors"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5819","DOI":"10.3390\/s110605819","article-title":"Design, fabrication and experimental validation of a novel dry-contact sensor for measuring electroencephalography signals without skin preparation","volume":"11","author":"Liao","year":"2011","journal-title":"Sensors"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kim, D., Yeon, C., Chung, E., and Kim, K. (2014, January 2\u20135). A non-invasive flexible multi-channel electrode for in vivo mouse EEG recoding. Proceedings of the IEEE Sensors Conference, Valencia, Spain.","DOI":"10.1109\/ICSENS.2014.6985200"},{"key":"ref_27","unstructured":"Franklin, K.B.J., and Paxinos, G. (1997). The Mouse Brain in Stereotaxic Coordinates, Academic Press."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"804","DOI":"10.1038\/nn.3710","article-title":"Cellular origins of auditory event-related potential deficits in Rett syndrome","volume":"17","author":"Goffin","year":"2014","journal-title":"Nat. Neurosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1023\/B:NERE.0000023605.68408.fb","article-title":"The effects of ketamine vary among inbred mouse strains and mimic schizophrenia for the P80, but not P20 or N40 auditory ERP components","volume":"29","author":"Connolly","year":"2004","journal-title":"Neurochem. Res."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"902","DOI":"10.1016\/j.visres.2005.09.006","article-title":"The visual evoked potential in the mouse\u2014Origins and response characteristics","volume":"46","author":"Ridder","year":"2006","journal-title":"Vis. Res."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3071","DOI":"10.1016\/S0042-6989(99)00022-X","article-title":"The visual physiology of the wild type mouse determined with pattern VEPs","volume":"39","author":"Porciatti","year":"1999","journal-title":"Vis. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/S0042-6989(96)00172-1","article-title":"Temporal aspects of contrast visual evoked potentials in the pigmented rat: Effect of dark rearing","volume":"37","author":"Pizzorusso","year":"1997","journal-title":"Vis. Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1034","DOI":"10.1109\/TBME.2004.827072","article-title":"BCI2000: A general-purpose brain-computer interface (BCI) system","volume":"51","author":"Schalk","year":"2004","journal-title":"IEEE Trans. Biomed. Eng."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/0361-9230(93)90319-7","article-title":"Flash and pattern reversal visual evoked potentials in C57BL\/6J and B6CBAF1\/J mice","volume":"32","author":"Strain","year":"1993","journal-title":"Brain Res. Bull."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/S0166-4328(01)00351-5","article-title":"Modulation of flash stimulation intensity and frequency: Effects on visual evoked potentials and oscillatory potentials recorded in awake, freely moving mice","volume":"131","author":"Lopez","year":"2002","journal-title":"Behav. Brain Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"825","DOI":"10.1016\/0361-9230(83)90215-0","article-title":"Hypothermia and chloropent anesthesia differentially affect the flash evoked potentials of hooded rats","volume":"10","author":"Dyer","year":"1983","journal-title":"Brain Res. Bull."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/0028-3908(81)90028-9","article-title":"Dose effects of pentobarbital on evoked potentials in visual cortex and superior colliculus of the albino rat","volume":"20","author":"Hetzler","year":"1981","journal-title":"Neuropharmacology"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s10633-010-9216-3","article-title":"Repeatability of short-duration transient visual evoked potentials in normal subjects","volume":"120","author":"Tello","year":"2010","journal-title":"Doc. Ophthalmol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1136\/bmj.331.7521.903","article-title":"Standard deviations and standard errors","volume":"331","author":"Altman","year":"2005","journal-title":"BMJ"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.jneumeth.2003.10.009","article-title":"EEGLAB: An open source toolbox for analysis of single-trial EEG dynamics including independent component analysis","volume":"134","author":"Delorme","year":"2004","journal-title":"J. Neurosci. Methods"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1109\/5.30749","article-title":"Time-frequency distributions\u2014A review","volume":"77","author":"Cohen","year":"1989","journal-title":"Proc. IEEE"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1109\/78.124950","article-title":"Kernel design for reduced interference distributions","volume":"40","author":"Jeong","year":"1992","journal-title":"IEEE Trans. Signal Process."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1109\/TBME.2004.824133","article-title":"Characterization of event related potentials using information theoretic distances measures","volume":"51","author":"Aviyente","year":"2004","journal-title":"IEEE Trans. Biomed. Eng."}],"container-title":["Sensors"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/2\/326\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T18:27:50Z","timestamp":1760207270000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1424-8220\/17\/2\/326"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,2,9]]},"references-count":43,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,2]]}},"alternative-id":["s17020326"],"URL":"https:\/\/doi.org\/10.3390\/s17020326","relation":{},"ISSN":["1424-8220"],"issn-type":[{"value":"1424-8220","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,2,9]]}}}